GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:15215498

Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvement.

Human R304Q disease alleles abolish recombinant CHST3 activity and alter patient fibroblast/urinary chondroitin sulfation. Soluble WT human CHST3 directly performs GalNAc-6 transfer.

PMID:9714738

Molecular cloning and expression of human chondroitin 6-sulfotransferase.

The study cloned human C6ST and established its relationship to chicken C6ST and broad tissue expression; the original enzyme-activity annotations are independently corroborated by later human assays.

PMID:9883891

Functional expression and genomic structure of human chondroitin 6-sulfotransferase.

Human soluble recombinant CHST3 catalyzes chondroitin GalNAc-6 sulfation with acceptor-sequence selectivity and also uses corneal keratan sulfate.

Reactome:R-HSA-2018682

CHST3,7 transfer SO4(2-) to position 6 of GalNAc on chondroitin chains

The normal reaction assigns GalNAc-6 sulfation to the CHST3/CHST7 Golgi catalyst set.

Reactome:R-HSA-2022854

Keratan sulfate biosynthesis

The keratan pathway includes distinct Gal and GlcNAc sulfation steps; general pathway membership does not identify which residue CHST3 modifies.

Reactome:R-HSA-2022870

CS-GAG biosynthesis

The pathway models formation and modification of chondroitin glycosaminoglycan chains, including the direct CHST3 sulfation step.

Reactome:R-HSA-2046222

CHST2,3,5,6 transfer sulfate to GlcNAc on keratan chain

The event explicitly includes CHST3 in a GlcNAc-sulfating set. Its target-specific chemical attribution remains unresolved despite the explicit curated membership.

Reactome:R-HSA-3595175

Defective CHST3 does not transfer SO4(2-) to chondroitin

The disease event models loss of the normal reaction by CHST3 mutants, while retaining Golgi compartment assignment.

PMID:11042394

Functional expression and genomic structure of human N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to beta-N-acetylglucosamine at the nonreducing end of an N-acetyllactosamine sequence.

A human GlcNAc-6 sulfotransferase transfers sulfate to terminal beta-linked GlcNAc acceptors; the abstract does not establish a CHST3-specific assay.

PMID:10491328

Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue.

Human and mouse intestinal GlcNAc-6 sulfotransferase homologs were cloned and a COS-cell assay detected GlcNAc-6 sulfation.

PMID:12218059

Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate.

Human corneal and mouse intestinal enzymes, but not the human intestinal homolog, act on longer poly-N-acetyllactosamine substrates; a four-enzyme pathway reconstructs a keratan-sulfate unit.

file:human/CHST3/CHST3-source-evidence.json

CHST3 source identities, substrate scope and review provenance

Compact authored source-access and substrate-scope record; primary evidence is cited directly in the annotations.

file:human/CHST3/CHST3-uniprot.txt

Fetched UniProt Q7LGC8 CHST3 record