NCU06005: ProtNLM localization review

Accession: Q7S2F2; original locus: NCU06005. Source method: ProtNLM2. Source version: live API snapshot 2026-09-09T03:00:51.831347+00:00.

The complete prediction record is preserved in NCU06005-protnlm-source.json; claim metadata are also preserved in NCU06005-protnlm-provenance.json. Original UniProt SL identifiers are retained; these are not substituted with GO terms.

Claim 1: SL-0086

Original text: Cytoplasm

Original evidence:

[
  {
    "evidenceCode": "ECO:0008006",
    "id": "ProtNLM2",
    "properties": [
      {
        "key": "model_score",
        "value": "1.00"
      },
      {
        "key": "string_match_text",
        "value": "GO:0005739"
      },
      {
        "key": "string_match_location",
        "value": "GO"
      },
      {
        "key": "string_match_type",
        "value": "hydrated"
      }
    ],
    "source": "Google"
  }
]

Assessment: UNC (confidence score 1).

Cytoplasm is plausible from the glycerol-kinase-specific target family and classical N. crassa enzymology identifying cytosolic glycerokinase in glycerol dissimilation (PMID:6284716). However, glycerol-kinase localization varies among homologs, and the experiment predates mapping to NCU06005. The frozen target annotation is mitochondrion, with no equivalent cytoplasm annotation. Because the locus correspondence is not established, this evidence does not decisively validate or refute the target location. The historical glp-4-to-NCU06005 mapping remains incomplete, so an exclusive cytosolic location and absence of a mitochondrial pool are not claimed. The source evidence hydrates Cytoplasm from GO:0005739, which denotes mitochondrion; that mismatch is retained as a provenance issue and is not treated as biological validation.

Evidence:

• file:NEUCR/NCU06005/NCU06005-uniprot.txt: “DR InterPro; IPR005999; Glycerol_kin.”
• PMID:6284716(https://pubmed.ncbi.nlm.nih.gov/6284716/): “Evidence from the enzymatic characterization of these
  mutants indicated that glp-2 and glp-4 were the structural genes encoding the
  mitochondrial glycerol-3-phosphate dehydrogenase and cytosolic glycerokinase,
  respectively.”

COR denotes a biologically supported location newly specified relative to the frozen target annotation snapshot. LSP denotes a less precise location than an established target annotation. Neither category establishes model training-set membership.