Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automated transfer of experimentally-verified manual GO annotation data to mouse-rat orthologs
Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs
Combined Automated Annotation using Multiple IEA Methods
Identification of a new glycerol-3-phosphate acyltransferase isoenzyme, mtGPAT2, in mitochondria.
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An NEM-sensitive GPAT activity, distinct from GPAT1, was detected in liver mitochondria of GPAT1-null mice and named mtGPAT2 (before the gene was cloned).
"we detected a novel NEM-sensitive GPAT activity in mitochondrial fractions and an anti-mtGPAT immunoreactive protein in liver mitochondria, but not in microsomes."
Molecular cloning of a murine glycerol-3-phosphate acyltransferase-like protein 1 (xGPAT1).
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Independent cloning of mouse Gpat2 (as xGPAT1), 801 aa, membrane-associated, with GPAT activity in CHO-K1 cells.
"xGPAT1 was distributed in the membrane fraction and showed GPAT activity when epitope-tagged xGPAT1 was expressed in Chinese hamster ovary (CHO)-K1 cells."
Cloning and functional characterization of a novel mitochondrial N-ethylmaleimide-sensitive glycerol-3-phosphate acyltransferase (GPAT2).
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Cloned from mouse testis; NEM-sensitive GPAT activity, mitochondrial location, and increased TAG synthesis in transfected Cos-7 cells; testis-enriched expression.
"When Cos-7 cells were transiently transfected with GPAT2, NEM-sensitive GPAT activity increased 30%."
Thematic review series: glycerolipids. Mammalian glycerol-3-phosphate acyltransferases: new genes for an old activity.
Membrane topology of murine glycerol-3-phosphate acyltransferase 2.
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GPAT2 is an integral membrane protein with two transmembrane segments; N- and C-termini and catalytic motifs I-III face the cytosol.
"These results suggest that the GPAT2 protein has two transmembrane segments and that the N-terminal and C-terminal regions of this protein face the cytoplasm."
Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols.
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GPAT2 prefers arachidonoyl-CoA; overexpression increases GPAT and AGPAT activity with arachidonoyl-CoA, PA synthesis, and arachidonate-rich TAG; expressed in primary spermatocytes.
"we have identified mitochondrial GPAT2 as an acyltransferase that prefers arachidonoyl-CoA."
GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells.
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GPAT2 binds MILI and is required for primary piRNA production in germline stem cells, independently of its acyltransferase activity.
"These data suggest that the acyltransferase activity of GPAT2 is dispensable for the biogenesis of piRNA."
Methylation of the Gpat2 promoter regulates transient expression during mouse spermatogenesis.
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Gpat2 expression peaks at 15 dpp, restricted to pachytene spermatocytes, and is controlled by promoter methylation and retinoic acid.
"Gpat2 mRNA content and protein expression were maximal at 15 dpp (days post-partum) and were restricted to pachytene spermatocytes."
Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis.
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In vivo testicular shRNA silencing of Gpat2 causes pachytene arrest and germ cell apoptosis (downstream phenotype; not annotated as process participation).
"Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage."
GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice†.
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Gpat2-deficient mice have defective piRNA production, retrotransposon derepression in fetal testes, and germ cell apoptosis.
"The deficient mice showed defective piRNA production and subsequent de-silencing of IAP and Line-1 retrotransposons in fetal testes."
UniProtKB Q14DK4 (GPAT2_MOUSE) record