PA4921 / ChoE (Q9HUP2) review notes

Literature

Search (Europe PMC REST, 2026-11-05: TITLE:"acetylcholinesterase" AND TITLE:aeruginosa)
returned PMID:22192836 and PMID:36753877; the PubMed search API was failing that day.

  1. Sanchez et al. 2012 PMID:22192836, abstract only. PA4921 is the gene for the
    cholinesterase (ChoE) activity of PAO1, an SGNH-hydrolase with a signal peptide
    PMID:22192836.
    Acetylthiocholine and propionylthiocholine are substrates; butyrylcholine inhibits;
    the purified recombinant enzyme behaves like mammalian AChE kinetically. The authors
    propose an acetylcholinesterase or propionylcholinesterase.
  2. Inoue et al. 2023 PMID:36753877, abstract only. Recombinant PAO1 AChE also catalyses
    acetyl transfer to amines and alcohols (aminolysis/alcoholysis) using acetylcholine or
    ethyl acetate as donor; a side activity of the acyl-enzyme.
  3. UniProt Q9HUP2 (unreviewed): SignalP 1-18; Pfam Lipase_GDSL; PANTHER PTHR45648; 13 PDB
    structures (6UQV-6UR1, 8D8W-8D91), mature chain 21-307.

The ChoE substrate choice (AChE vs propionylcholinesterase) is unresolved, so
GO:0004104 cholinesterase activity, the term the curator used, is the right level;
GO:0003990 acetylcholinesterase activity would overstate it.

PseudoCAP IBA-seed assessment

PTN node node term n_seeds leaf IBAs seed annotation (term, evidence, PMID) verdict rationale other seeds IBD stands without this seed?
PTN005219546 GO:0052689 carboxylic ester hydrolase activity 2 960 (51 taxa) GO:0004104 cholinesterase activity, IDA, PMID:22192836 (PseudoCAP) SOUND Purified recombinant enzyme hydrolyses acetyl- and propionylthiocholine and p-nitrophenyl esters (abstract). Cholinesterase is_a carboxylic ester hydrolase, so the seed supports the node term. EstA (O33407, PA5112): IMP PseudoCAP and IDA UniProt carboxylesterase (PMID:20931591) Yes; EstA's purified-enzyme esterase evidence alone supports it, and ester hydrolysis is the conserved GDSL/SGNH chemistry. Consistent with the estA review (SOUND). No GO_CENTRAL defect.

PTN005219546 also carries a GO:0008610 lipid biosynthetic process IBD seeded only by EstA
(not by PA4921). The node-level GO_CENTRAL defect for it is recorded once in
genes/PSEAE/estA/estA-ai-review.yaml; not repeated here. On PA4921 the propagated row
is removed: a periplasmic/secreted cholinesterase has no role in lipid biosynthesis.

Annotation decisions