PHS1 (YJL097W; UniProt P40857) notes

Evidence journal (no paid deep research run; built from UniProt and cached publications).

Function

• Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase (HACD family), EC 4.2.1.134, essential [UniProt:P40857].
• PMID:18272525.
• Topology: PMID:18272525; essential residues Tyr-149, Glu-156.
• PMID:23416297.
• Sphingolipid consequence: PMID:18272525.

Location

• ER membrane (topology, HTP ER calls). Huh/Hazbun/Matsumoto HTP vacuole/vacuolar membrane calls kept as non-core.

YeastPathways issues

• PWY-5177 (glutaryl-CoA degradation) RCA rows: carboxylic acid catabolic process and cytosol removed; Phs1 is an ER anabolic (3R) enzyme. MF row kept.
• PWY-5080-1 uses EC 4.2.1.17 (enoyl-CoA hydratase, 3S beta-oxidation) for the dehydration step; correct EC is 4.2.1.134 -> GO:0102158.
• Vacuolar transport (IMP, Yu et al. 2006 screen) marked over-annotated PMID:16943325.