Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine.
-
DesI, the desosamine-pathway enzyme about 61% identical to EryCIV, is a PLP-dependent aminotransferase whose crystal structure was solved with its product, dTDP-4-amino-4,6-dideoxyglucose.
"The structure of DesI was solved in complex with its product, dTDP-4-amino-4,6-dideoxyglucose"
UniProtKB A4F7N3 (eryCIV) record - "NDP-6-deoxyhexose 3,4-dehydratase"; DegT/DnrJ/EryC1 family
-
EryCIV is a PLP-dependent DegT/DnrJ/EryC1-family enzyme named as an NDP-6-deoxyhexose 3,4-dehydratase.
"EryCIV NDP-6-deoxyhexose 3,4-dehydratase"
TreeGrafter-generated GO annotations
OpenScientist TreeGrafter stress test for eryCIV transaminase activity
-
Blinded run placed EryCIV on the transaminase side of the DegT/DnrJ/EryC1 family: catalytic PLP Schiff-base Lys200 conserved (ColD has His at this position) and DesI (Q9ZGH0) the nearest characterized neighbor; verdict partially supported / too general.
"EryCIV has Lys, not His"
-
The run recommends refining the term to a dTDP-sugar C4 aminotransferase rather than removing it.
"This is a true-positive IEA requiring a"