GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

PMID:12024217

Comparison of the genomes of two Xanthomonas pathogens with differing host specificities

Genome sequence paper for X. campestris pv. campestris strain ATCC 33913. Reports the gene XCC4213 (btuE) as part of the complete genome but does not provide functional characterization.

PMID:20621065

The Escherichia coli btuE gene, encodes a glutathione peroxidase that is induced under oxidative stress conditions.

Purified E. coli BtuE (P06610), which is in the same PANTHER subfamily and PAINT clade (PTN000173904) as Q8P365, reduces H2O2 and cumene, tert-butyl and linoleic acid hydroperoxides. It prefers thioredoxins A or C to glutathione as the reductant.

"Figure 2 shows that BtuE uses preferentially thioredoxins A or C as reducing agents irrespective of the peroxide substrate."
E. coli btuE expression is induced by oxidative-stress elicitors.

"The btuE gene was induced when cells were exposed to oxidative stress elicitors that included potassium tellurite, menadione and hydrogen peroxide"

file:XANCP/Q8P365/Q8P365-uniprot.txt

UniProt record for Q8P365 (btuE)

UniProt classifies Q8P365 as a glutathione peroxidase belonging to the glutathione peroxidase family, with an active site at position 37 and conserved GSHPx, thioredoxin-like fold, and GPX active site domains.

file:XANCP/Q8P365/Q8P365-deep-research-falcon.md

Falcon deep research for btuE (Q8P365)

Falcon deep research found no organism-specific experimental literature for Q8P365/btuE in X. campestris. All functional inference is based on domain architecture and glutathione peroxidase family membership. Bacterial GPx-family proteins generally function in peroxide detoxification and oxidative stress defense.

file:XANCP/Q8P365/Q8P365-bioinformatics/RESULTS-rereview.md

Q8P365 re-review bioinformatics (PANTHER tree placement and alignment to E. coli BtuE)

In the PANTHER 19 tree, Q8P365 descends from PTN000173904 (Gammaproteobacteria, SF40) together with E. coli BtuE. The Xanthomonas paralog Q8PAI2 arises from a lineage-specific duplication. Q8P365 is 49% identical to P06610, with conserved C37, C83, Q71, W143 and N144.