Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
UniProtKB record A0A0H2M6H0 (A0A0H2M6H0_VARPD) - Putative FMN reductase (NADPH) from Variovorax paradoxus TBEA6
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VPARA_09290 (ssuE, A0A0H2M6H0) is a 194-aa SsuE-family FMN reductase (NCBIfam TIGR03567, InterPro IPR020048, PANTHER PTHR43408:SF1, Pfam PF03358 residues 1-142).
"DR NCBIfam; TIGR03567; FMN_reduc_SsuE; 1."
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VPARA_09290 (ssuE) corresponds to EMBL CDS accession KLN57918.1 on genomic contig JZWI01000005, where consecutive CDS accessions and locus tags stepping by 10 place ssuE in the same organosulfonate gene cluster as ssuB2 (VPARA_09230, KLN57912.1), ssuC5 (VPARA_09240, KLN57913.1), ssuD (VPARA_09250, KLN57914.1), and ssuA2 (VPARA_09270, KLN57916.1).
"DR EMBL; JZWI01000005; KLN57918.1; -; Genomic_DNA."
Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli.
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Purified E. coli SsuE reduces FMN to FMNH2 and supplies the reduced flavin required by SsuD to cleave alkanesulfonates into sulfite and the corresponding aldehyde.
"SsuE is the FMN reducing enzyme providing SsuD with FMNH(2)."
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SsuD-catalyzed alkanesulfonate monooxygenation strictly requires reduced FMN and molecular oxygen.
"SsuD catalysis was absolutely dependent on FMNH(2) and oxygen"
The ssu locus plays a key role in organosulfur metabolism in Pseudomonas putida S-313.
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Heterologously expressed P. putida S-313 SsuE catalyzes NADPH-dependent FMN reduction in cell extracts.
"SsuE revealed NADPH-dependent FMN reductase activity and lower levels of FAD reductase activity (32% of the value with FMN). No activity was observed with NADH."
Exposing the Alkanesulfonate Monooxygenase Protein-Protein Interaction Sites.
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Optimal transfer of reduced flavin from SsuE to SsuD involves specific protein-protein interactions, while diffusional transfer can also support turnover under defined conditions.
"optimal transfer of reduced flavin from SsuE to SsuD requires defined protein-protein interactions, but diffusion can occur under specified conditions."