Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Sensing of cadmium and copper ions by externally exposed ADL, ASE, and ASH neurons elicits avoidance response in Caenorhabditis elegans.
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che-2 mutants lack chemotactic avoidance to Cd2+ and Cu2+ due to structural defects in ciliated neurons
"Mutants that have structural defects in ciliated neurons (che-2 and osm-3) as well as worms with three laser-operated neurons (ADL, ASE, and ASH), showed no avoidance behavior from Cd2+ and Cu2+"
A novel WD40 protein, CHE-2, acts cell-autonomously in the formation of C. elegans sensory cilia.
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CHE-2 is a WD40 repeat protein essential for cilium formation
"The che-2 gene encodes a new member of the WD40 protein family, suggesting that it acts in protein-protein interaction."
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che-2 mutants have extremely short cilia with abnormal posterior projection
"These mutants have extremely short cilia with an abnormal posterior projection, and show defects in behaviors that are mediated by ciliated sensory neurons."
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CHE-2::GFP localizes to cilia of almost all ciliated sensory neurons
"CHE-2-tagged green fluorescent protein is localized at the cilia of almost all the ciliated sensory neurons."
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CHE-2 acts cell-autonomously in cilium formation
"Expression of che-2 in a subset of sensory neurons of a che-2 mutant by using a heterologous promoter resulted in restoration of the functions and cilium morphology of only the che-2-expressing neurons. Thus, che-2 acts cell-autonomously."
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Both WD40 repeats and C-terminal domain are required for function
"Analysis of mutation sites showed that both the amino-terminal WD40 repeats and the carboxyl-terminal non-WD40 domain are necessary for the CHE-2 function."
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Cilium extension can be rescued even at adult stage by che-2 expression
"Expression of che-2 in a che-2 mutant under a heat shock promoter showed that the extension of cilia, surprisingly, can occur even at the adult stage, and that such cilia can function apparently normally in behavior."
DAF-7/TGF-beta expression required for the normal larval development in C. elegans is controlled by a presumed guanylyl cyclase DAF-11.
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che-2 is placed in genetic pathway between daf-11 and daf-7 for dauer formation
"cilium-related genes che-2 and che-3 are placed between daf-11 and daf-7, in the genetic pathway controlling dauer formation"
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Cilium-related genes affect TGF-beta signaling indirectly through sensory function
"Expression of daf-11 cDNA by cell specific promoters suggests that daf-11 acts cell autonomously in ASI chemosensory neurons for daf-7 expression."
Genetic analysis of chemosensory control of dauer formation in Caenorhabditis elegans.
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che-2 is one of nine cilium-structure genes affecting dauer formation
"Dauer-defective mutations in nine genes cause structurally defective chemosensory cilia, thereby blocking chemosensation"
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Cilium structure mutations block chemosensation required for dauer regulation
"Dauer-defective mutations in nine genes cause structurally defective chemosensory cilia, thereby blocking chemosensation"
The BBSome controls IFT assembly and turnaround in cilia.
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IFT-B components show active anterograde movement
"Similar to OSM-6, in dyf-2(jhu616), all other IFT-B components examined also showed active anterograde movements, but lost most of the retrograde IFT movements and accumulate at the ciliary tip"
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The BBSome assembles IFT complexes at the ciliary base
"the BBSome (refs 3, 4), a group of conserved proteins affected in human Bardet-Biedl syndrome(5) (BBS), assembles IFT complexes at the ciliary base, then binds to the anterograde IFT particle"
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IFT-A and IFT-B subcomplexes associate during anterograde transport
"IFT-A component CHE-11 and IFT-B component IFT-20 showed fluorescence complementation in either wild-type or dyf-2(jhu616), indicative of the association between IFT-A and IFT-B subcomplexes"
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IFT-B components accumulate at cilia tip when turnaround is defective
"all other IFT-B components examined also showed active anterograde movements, but lost most of the retrograde IFT movements and accumulate at the ciliary tip"
The Importance of cGMP Signaling in Sensory Cilia for Body Size Regulation in Caenorhabditis elegans.
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Cilium structure mutants including che-2 exhibit small body size
"The body size of Caenorhabditis elegans is thought to be controlled by sensory inputs because many mutants with sensory cilium structure defects exhibit small body size"
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Body size regulation depends on cGMP signaling in sensory cilia
"the cGMP level is precisely controlled by GCY-12 and PDE-2 to determine body size through EGL-4"
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Cilium defects disturb balanced control of cGMP levels
"the defects in the sensory cilium structure may disturb the balanced control of the cGMP level"
Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C. elegans Sensory Cilia.
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IFT-B complex is required for dynein-2 ciliary localization
"Disruption of the dynein-2 tail domain, light intermediate chain, or intraflagellar transport (IFT)-B complex abolishes dynein-2's ciliary localization, revealing their important roles in ciliary entry of dynein-2"
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IFT-A and IFT-B function in ciliary transport
"our affinity purification and genetic analyses show that IFT-A subunits IFT-139 and IFT-43 function redundantly to promote dynein-2 motility"
Multiple chemosensory defects in daf-11 and daf-21 mutants of Caenorhabditis elegans.
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daf-11 and daf-21 mutants have chemosensory defects including in chemotaxis
"daf-11 and daf-21 mutants are not defective in avoidance of certain non-volatile repellents, but are defective in taxis to non-volatile attractants"
Deep research report on che-2