Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
The barley Mlo gene: a novel control element of plant pathogen resistance.
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Positional cloning of Mlo; recessive mlo alleles confer leaf lesions and broad-spectrum powdery mildew resistance; Mlo proposed as a negative regulator of cell death and defense onset.
"Mutation-induced recessive alleles (mlo) of the barley Mlo locus confer a leaf lesion phenotype and broad spectrum resistance to the fungal pathogen, Erysiphe graminis f. sp. hordei."
Topology, subcellular localization, and sequence diversity of the Mlo family in plants.
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MLO has 7 TM helices (N-terminus outside, C-terminus inside) and is in the plasma membrane.
"Mlo is membrane-anchored by 7 transmembrane (TM) helices such that the N terminus is located extracellularly and the C terminus intracellularly."
Calmodulin interacts with MLO protein to regulate defence against mildew in barley.
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Ca2+-dependent calmodulin binding domain; loss of CaM binding halves MLO activity; MLO acts independently of heterotrimeric G proteins.
"MLO seems to function independently of heterotrimeric G proteins."
The barley MLO modulator of defense and cell death is responsive to biotic and abiotic stress stimuli.
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Wild-type MLO dampens penetration-site H2O2 burst and suppresses mesophyll cell death; Mlo transcript is induced by biotic and abiotic stresses.
"Mlo transcript abundance increases in response to Bgh, rice (Oryza sativa) blast, wounding, paraquat treatment, a wheat powdery mildew-derived carbohydrate elicitor, and during leaf senescence."
Recruitment and interaction dynamics of plant penetration resistance components in a plasma membrane microdomain.
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MLO accumulates in a pathogen-triggered plasma membrane microdomain beneath appressoria; MLO-CaM FRET increases at penetration sites.
"Our data provide evidence for the formation of a pathogen-triggered PM microdomain"
Conserved extracellular cysteine residues and cytoplasmic loop-loop interplay are required for functionality of the heptahelical MLO protein.
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Conserved extracellular cysteines and the C-terminal tail are required for MLO function; MLO dimerizes/oligomerizes in planta.
"FRET (fluorescence resonance energy transfer) analysis revealed evidence for in planta MLO dimerization/oligomerization."
Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley mlo protein.
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Second and third cytoplasmic loops are critical for susceptibility-conferring activity; biochemical activity of MLO unknown.
"Mlo codes for a member of a plant-specific family of polytopic integral membrane proteins with unknown biochemical activity."
RALF signaling pathway activates MLO calcium channels to maintain pollen tube integrity.
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Arabidopsis MLO1/5/9/15 act as Ca2+ channels in pollen tubes (paralogs, not barley MLO).
"We identified MILDEW RESISTANCE LOCUS O (MLO) family proteins MLO1, 5, 9, 15, as Ca2+ channels required for Ca2+ influx and pollen tube integrity."
mlo-Based Resistance: An Apparently Universal "Weapon" to Defeat Powdery Mildew Disease.
Deep research report on barley MLO (falcon)