Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:22692205

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.

Establishes the intracellular vertebrate pathway for degrading sialic acids with N-glycolyl groups (Neu5Gc), the biological context for NPL's role in sialic acid catabolism and Neu5Gc degradation in human cells.

PMID:25416956

A proteome-scale map of the human interactome network.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:33895133

The metalloprotein YhcH is an anomerase providing N-acetylneuraminate aldolase with the open form of its substrate.

Purified recombinant human NPL cleaves Neu5Ac, and its activity is stimulated by the anomerases NanM and YhcH that supply the open (linear) form of the substrate; basis for the EC 4.1.3.3 catalytic-activity and IDA annotations.

Reactome:R-HSA-4085217

NPL cleaves Neu5Ac,Neu5Gc to ManNAc,ManNGc and pyruvate

Cytosolic tetrameric NPL cleaves both Neu5Ac and Neu5Gc to ManNAc/ManNGc plus pyruvate; source of the cytosol localization and support for Neu5Gc degradation.

PMID:37390204

N-acetylneuraminate pyruvate lyase controls sialylation of muscle glycoproteins essential for muscle regeneration and function.

Deleterious NPL variants cause skeletal myopathy and cardiac edema (human and zebrafish); NPL loss raises free sialic acid and disrupts muscle glycoprotein sialylation, and oral N-acetylmannosamine rescues the myopathy in mouse models.