Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteins.
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Survey of tagged human and yeast DHHC proteins; most localize to the ER and/or Golgi.
"Most DHHC proteins are localized in the ER and/or Golgi, with a few localized in the plasma membrane and one in the yeast vacuole."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.
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ZDHHC23 (with ZDHHC22) palmitoylates the BK channel S0-S1 loop and promotes its surface expression; ZDHHC23 localizes mainly to Golgi/TGN.
"palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23."
A reference map of the human binary protein interactome.
UniProt record for human ZDHHC23
S-Acylation controls functional coupling of BK channel pore-forming α-subunits and β1-subunits.
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Genetic deletion of mouse Zdhhc23 reduces endogenous BK channel alpha-subunit S-acylation in vascular smooth muscle; the functional consequence depends on beta1 subunits.
"S-acylation is significantly reduced in mice with a genetic deletion of the palmitoyl acyltransferase (Zdhhc23) that controls S-acylation of the S0-S1 loop."
DHHC protein family targets different subsets of glioma stem cells in specific niches.
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ZDHHC18 and ZDHHC23 interact with RNF144A and regulate BMI1 polyubiquitination in glioma stem cells; direct BMI1 S-acylation was not shown.
"ZDHHC18 and ZDHHC23 competitively interact with a BMI1 E3 ligase, RNF144A, to regulate the polyubiquitination and accumulation of BMI1."
GFAP palmitoylcation mediated by ZDHHC23 in spinal astrocytes contributes to the development of neuropathic pain.
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In a mouse cancer-pain model ZDHHC23 upregulation in spinal astrocytes is associated with increased GFAP palmitoylation.
"palmitoyltransferase ZDHHC23 expression was upregulated, leading to increased palmitoylation levels of GFAP"