Deep research: just deep-research-falcon ARATH PIN2 failed (Edison API 429 rate limit, 2026-10-05); review based on cached publications and UniProt/GOA.
This dated audit supersedes conflicting earlier shorthand. All 34 original annotation payloads, including qualifiers and inherited NEW rows, were retained exactly. Every row was reconsidered against the target biology and cited sources. Actual Falcon and fallback provider attempts failed; the orchestration logs are preserved in .cache/module-audit-logs/auxin-polar-fetch/provider-publication-results.json. Existing provider reports, where present, served only as discovery aids. Manual primary-source inspection and normally fetched caches support the decisions below. No provider report was fabricated.
Native transport includes apical epidermal/lateral-root-cap and basal young-cortical membrane pools; both are core locations rather than calling the less familiar cortical orientation peripheral. Tissue direction must not be confused with the molecular export direction. The inherited NEW apical-location payload is preserved. The PIN1 structure supports a stated homodimerization inference for PIN2, not a direct PIN2 oligomeric measurement.
Full PMID:28698356 was recovered at publications/url_httpspmc.ncbi.nlm.nih.govarticles_PMC5580755report_xml.md. The source does more than measure PIN abundance: “neither hypoxia nor hypoxia in the presence of α-NAA further increased the angle at which pin2-1 roots grew”. This supports the contextual hypoxic growth response, not oxygen-sensing activity.
The actual PTHR31752 PAINT table contains ER and PM assertions at PTN001589272, including PIN5/6/8 as ER experimental seeds. PIN2 ER remains UNDECIDED rather than rejected from long-PIN/PM predominance alone; no target-resolved negative or demonstrated loss of every ER pool was found. The chloroplast prediction also remains UNDECIDED: root expression and plasma-membrane targeting do not exclude plastids or a minor additional pool. No new root-organelle narrowing is applied.
Final actions: 16 ACCEPT, 5 KEEP_AS_NON_CORE, 4 MODIFY, 1 NEW, 6 REMOVE, 2 UNDECIDED. This panel contains no negated source rows; there are no manufactured NOT assertions. Exact-row payload proof and focused validation are recorded in .cache/module-audit-logs/auxin-polar-six-*. Reciprocal scientific consultation with the parallel annotation reviewer confirmed source-specific localization, integral binding and substrate-assay distinctions.
The AtSubP GO_REF:0000122 ISM location prediction is now MARK_AS_OVER_ANNOTATED. It was assessed as a computational compartment assignment against positive target localization and carrier architecture, rather than deferred under the rule for inaccessible experimental papers. PMID:9843496 supports native polar root-cell membrane localization; curated UniProt localization is experimentally tied to PMID:20439545/33705718. The predicted first helix at residues 8–28 is consistent with an N-terminal membrane anchor, not positive evidence for chloroplast import. Neither a missing TRANSIT feature nor this helix proves that all plastid-envelope targeting is impossible; no blanket transit-peptide requirement is asserted. This revises the earlier unresolved judgment without inventing an exclusion assay or asserting loss of a phylogenetically inherited location. All original row payloads, flags, evidence codes and raw source files are preserved; no new annotation is proposed.