Use of the ND evidence code for Gene Ontology (GO) terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for MTC7_YEAST
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Mtc7 is a small predicted multi-pass membrane protein with two transmembrane helices and no established molecular function.
"FUNCTION: May be involved in telomere capping. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TRANSMEM features are annotated at residues 13-33 and 42-62."
yeast/MTC7/MTC7-bioinformatics/RESULTS.md
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Local topology analysis recovered a hydrophobic segment overlapping the second UniProt TRANSMEM feature but did not independently recover both annotated helices.
"The Kyte-Doolittle scan generated by tm_analysis_results.json predicted one segment, residues 45-63, which overlaps UniProt's second TRANSMEM feature but did not independently recover TM1."
A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae
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MTC7 was named as a maintenance-of-telomere-capping gene from a genome-wide cdc13-1 genetic interaction screen.
"We also identified a number of genes of previously unknown function that we term RTC, for restriction of telomere capping, or MTC, for maintenance of telomere capping."
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The evidence is genetic and phenotypic rather than a direct molecular demonstration of Mtc7 function at telomeres.
"We identified 369 gene deletions, in eight different phenotypic classes, that reproducibly demonstrated subtle genetic interactions with the cdc13-1 mutation."
A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.
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YEL033W/MTC7 was the one candidate retested in the 2004 genome-wide deletion screen whose short-telomere phenotype did not cosegregate with the YEL033W deletion marker.
"The single exception, yel033w , showed a short telomere phenotype that segregated independently from the antibiotic resistance."
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The authors concluded that yel033w deletion segregated with very slow growth while short telomeres segregated with a suppressor of that growth phenotype.
"the yel033w deletion segregates with a very slow growth phenotype and the short telomeres segregate with a suppressor mutation responsible for restored growth"
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Askree et al. retained YEL033W as a candidate genetic interactor of an unidentified telomere-function gene, not as a demonstrated telomere-length gene.
"YEL033W , a dubious ORF, appears to genetically interact with an unidentified gene with telomere function"
Falcon deep research report for MTC7
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Falcon found limited direct mechanistic literature for MTC7 and highlighted that the available evidence is genetic and phenotypic rather than mechanistic.
"MTC7 is a poorly characterized gene with limited direct mechanistic literature; current interpretation relies on genetic association plus modern telomere-capping context rather than a definitive molecular mechanism."