Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProt entry Q93XQ5 for Nicotiana attenuata PMT1
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UniProt identifies Q93XQ5 as Putrescine N-methyltransferase 1
"DE RecName: Full=Putrescine N-methyltransferase 1"
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UniProt assigns PMT1 to nicotine biosynthesis
"CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis."
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UniProt reports predominant root expression for PMT1
"CC -!- TISSUE SPECIFICITY: Mainly expressed in roots."
NaPMT1.1 literature review notes
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PMT1 is the reviewed Nicotiana attenuata PMT1 pathway enzyme
"UniProt curates Q93XQ5 as Putrescine N-methyltransferase 1 and places it in nicotine biosynthesis with predominant root expression."
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PMT catalyzes the first dedicated pyrrolidine-branch step toward nicotine
"PMT catalyzes S-adenosylmethionine-dependent methylation of putrescine to N-methylputrescine, the first specific metabolite on the route to nicotine."
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Spermidine synthase annotations on PMT1 are evolutionary-family overcalls
"TreeGrafter assignment to spermidine synthase reflects PMT's evolutionary origin from spermidine synthase rather than its present catalytic activity."
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PMT1 is part of jasmonate-, wound-, and herbivory-inducible nicotine defense
"In N. attenuata, Winz and Baldwin cloned PMT1 and PMT2 and found that both root PMT transcripts rose after MeJA, wounding, and Manduca sexta herbivory, while ethylene suppressed this induction."
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PMT1 fits the duplicated root nicotine pathway described in the Nicotiana genome study
"The 2017 Nicotiana genome paper places root nicotine biosynthesis in N. attenuata as a duplicated specialization of polyamine and NAD metabolism, consistent with PMT as a root defense-pathway enzyme."
Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host Nicotiana attenuata. IV. Insect-Induced ethylene reduces jasmonate-induced nicotine accumulation by regulating putrescine N-methyltransferase transcripts.
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PMT1 and PMT2 were cloned from Nicotiana attenuata as likely rate-limiting nicotine-pathway genes
"we cloned the putrescine methyltransferase genes (NaPMT1 and NaPMT2) of N. attenuata, which are thought to represent the rate limiting step in nicotine biosynthesis"
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Root PMT transcripts increase after methyl jasmonate treatment
"Transcripts of both root putrescine N-methyltransferase (PMT) genes and nicotine accumulation increased dramatically within 10 h of shoot MeJA treatment"
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Wounding and Manduca sexta herbivory elevate PMT transcript accumulation
"1-MCP pretreatment dramatically amplified the transcript accumulation resulting from both wounding and M. sexta herbivory"
Putrescine N-methyltransferase--the start for alkaloids.
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PMT catalyzes S-adenosylmethionine-dependent methylation of putrescine
"Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM) dependent methylation of the diamine putrescine."
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N-methylputrescine is the first specific metabolite on the route to nicotine
"The product N-methylputrescine is the first specific metabolite on the route to nicotine, tropane, and nortropane alkaloids."
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PMT evolved from plant spermidine synthase
"PMT is likely to have evolved from the ubiquitous enzyme spermidine synthase."
Wild tobacco genomes reveal the evolution of nicotine biosynthesis.
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Root nicotine biosynthesis in Nicotiana evolved from duplicated polyamine and NAD pathways
"The biosynthetic machinery that allows for nicotine synthesis in the roots evolved from the stepwise duplications of two ancient primary metabolic pathways: the polyamine and nicotinamide adenine dinucleotide (NAD) pathways."