Standard just fetch-gene PSEPK holD --uniprot-id Q88Q02 seeded zero GOA rows. The pristine document/raw hashes were frozen in pseudomonas-new-members-baseline.json before editing. Default Falcon was attempted and failed HTTP403; no provider content was fabricated.
The current UniProt label Energy transducer TonB is a ProtNLM prediction and is contradicted by primary source mapping. PMID21955458 explicitly cloned KT2440 holC and holD. Its official Additional file9 forward/reversepsi primers map uniquely to PP_0696, not PP_0978. See ../holC/holC-source-analysis for source PDF, unmodified extraction, genomic coordinates, corrected-start calculation and executable check. The older291-residue protein matches the official sequence. The corrected TTG native start predicts261 residues; the GST expression insert begins at the following CTG codon and covers database residues32–291 (260native residues). GST was cleaved during purification. Do not claim the full older291-residue protein or its upstream30-residue extension was assayed.
PMID:21955458 directly measures low-salt ssDNA binding by KT2440chi–psi. Attribution to psi comes from chimeric and deletion experiments on P. aeruginosa; complex-level qualifier contributes_to is therefore retained for the KT2440 binding row. Its in vivo importance remains a question, not a second conserved core assertion. PMID:16210315 discovers endogenous P. aeruginosa psi and demonstrates stimulation of the minimal replicase. PMID:17210572 dissects E. coli psi stabilization of the ATP-induced loader state. These comparative data support the loader-contribution core, explicitly distinguished from direct KT2440 chemistry.
Three NEW rows: ISS contributes_to clamp loader; IDA contributes_to ssDNA binding of the complex; IC DNA replication. The BP is direct structural machinery participation. Current QuickGO comparator queries returned5 replication annotations each for E. coli HolA/P28630, HolB/P28631 and HolC/P28905. GO0006260 parent check and empty Q88Q02 GO-CAM-index lookup found no convention conflict or redundant proposed BP. No transporter, TonB, origin-firing-regulation, nuclease or polymerase activity is asserted.
No PANTHER/InterPro membership was present in the frozen target record; do not invent a family identifier. Use a descriptive divergent pseudomonad psi family with the actual reviewed gene and the explicit sequence/start limitation.
All historical annotation payloads, inherited NEW proposals, and frozen raw/provider files are preserved. Source confidence distinguishes direct target assays from comparative Pseudomonas evidence. No publication cache was refreshed.
The earlier use of “inherited NEW” described inheritance from a review-round snapshot, not upstream main or machine GOA. Independent git ancestry and the pristine seed baseline establish that the HolD replication and Bacillus DnaB DNA-binding proposals were authored in 47acac27a0. Their complete initial and pre-revision rows are preserved in analysis/module_audits/bacterial_chromosomal_dna_replication/round2-authored-proposal-revisions.yaml; only the two explicitly authorized authored proposals are revised. The pristine seed baseline, all machine payloads and raw/source files remain frozen. No native N-terminal measurement is newly asserted.