Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV.
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Reconstituted ParC+ParE decatenates and relaxes DNA without supercoiling.
"The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity."
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Activity requires ATP, Mg2+ and both subunits.
"Enzymatic activity was dependent on ATP, Mg 2+ (data not shown), and the presence of both subunits"
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In type IIA enzymes the A subunit cleaves and rejoins DNA; the B subunit is the ATPase.
"GyrA is responsible for the DNA strand binding, cleavage, and rejoining, and GyrB is responsible for ATPase activity."
UniProt entry Q9HUK1 (PARC_PSEAE)