ENTPD1 original evidence and access guide

The review distinguishes native human enzyme preparations, human recombinant constructs, other species and the six source-described natural products. No new publication quotations are added. “Original read” below means the listed operative text and captions were inspected; it does not mean figure pixels were independently reanalyzed. Normal publication-cache availability flags are distinct from this actual reviewer access.

Direct enzyme evidence

Human localization and interaction

Placental products

Localization datasets with remaining gaps

Donor evidence and source interpretation

Database and product provenance

PANTHER family PTHR11782 places human P49961 at PTN002483056 in ENTPD1 subfamilySF32. Six imported IBA assertions match actual IBD nodes: PTN000207579 for GDP/UDP/NTP activities, PTN000925965 for plasma membrane, and PTN000746626 for NDP/NTP catabolism. Target descent and the exact donor sets were checked against the pinned IBD; no matching NOT record occurs on the target path. A target self-donor is valid experimental grounding.

Reactome NTP event8850846 and NDP event8850854 were traced through catalysts8850850/8850852 and complexes8850845/8850847 to human P49961 protein913566. Calcium/magnesium is a set of alternatives. Rhea aggregate NTP-to-NMP chemistry releases two inorganic phosphates; component reactions and substrate-specific evidence are distinguished. InterPro IPR000407 supports the broad family hydrolase mapping. Current inaccessible or unmatched ARBA rules do not override independent experiments.

The six imported products remain P49961-1(510),-2(517),-3(306),-4(372),-5(402),-6(522). All reconstruct exactly from UniProt P49961 alternative sequences and independently downloaded FASTA records. Coordinate projection establishes sequence differences, not activity, topology in a particular cell or equivalence to artificial constructs. The short placental product lacks the unchanged canonical C-terminal membrane-spanning segment; products4/5 lack the unchanged canonical N-terminal segment. Retaining one catalytic residue does not establish catalytic competence.

Core synthesis and reproducible products

One apyrase unit represents successive extracellular ATP/ADP hydrolysis with phosphate release, class-level nucleotide catabolism and the demonstrated contribution to negative regulation of platelet aggregation. The external side of the plasma membrane is the specific core location supported by the human surface evidence. The independently read originals are PMID:8529670 (printed pp. 67, 70–71; Table 3), PMID:8996251 (Methods, Fig. 1 and human surface Fig. 5), and PMID:10636909 (Methods, Fig. 4 and Fig. 5 Results). Native placental enzyme was not isoform resolved; endothelial human and porcine experiments remain distinguished. A separate CD73 reaction produces adenosine. The product-analysis bundle supplies exact replay inputs and coordinate projections for all six source-described human products; these projections do not establish functional equivalence.

Follow-up evidence boundaries

PMID:17502665(https://doi.org/10.1084/jem.20062512), official PMC Results sections3–5/Figures3–5 and Methods12–15, connects mouse CD39-dependent nucleotide degradation to CD73-mediated adenosine generation, A2A-dependent responses, proliferation and graft suppression. This supports the immune-regulatory context while leaving AMP hydrolysis with CD73. Human/primate observations in that paper remain unpublished.

PMID:16478441(https://doi.org/10.1042/BJ20051568), Results and Figures1–3, supports human CD39–RanBPM association and identifies CD39 as the enzyme whose ATP/ADP hydrolysis is reduced by RanBPM. It does not establish CD39 as an adapter or an enzyme regulator. The review retains the supported association as non-core under the campaign instructions.

The CDP/UDP questions identify measurements missing from the inspected human substrate panel and the partially accessible rat original; they do not erase the accepted PAINT UDP assertion or claim that the entire literature lacks those measurements.