RHOJ: exact selected product and same-gene reference

Selected G3V4H1 has 153 residues; reference Q9H4E5 has 214 residues.

Biopython PairwiseAligner global BLOSUM62, gap open -10, extension -0.5.

Reference interval Selected interval Identical residues
1–103 1–103 103
115–115 104–104 1
121–127 105–111 3
160–181 112–133 5
182–184 140–142 2
195–201 143–149 2
205–208 150–153 1

Reference feature mapping (sequence coverage does not establish retained function):

Reference feature Interval Mapped / length Identical
Motif: Effector region 50–58 9 / 9 9
Binding site: 31–31 1 / 1 1
Binding site: 33–33 1 / 1 1
Binding site: 34–34 1 / 1 1
Binding site: 35–35 1 / 1 1
Binding site: 35–35 1 / 1 1
Binding site: 36–36 1 / 1 1
Binding site: 50–50 1 / 1 1
Binding site: 53–53 1 / 1 1
Binding site: 53–53 1 / 1 1
Binding site: 75–75 1 / 1 1
Binding site: 78–78 1 / 1 1
Binding site: 136–136 0 / 1 0
Binding site: 177–177 1 / 1 0

A shared segment does not establish that a truncated product folds, is expressed, localizes, or catalyzes the full-length reaction.

Inputs are the sibling frozen ProtNLM source and same-gene reference JSON files. Reproduce with UV_NO_SYNC=1 uv run python compare_sequences.py from this directory.