Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
UniProtKB entry Q88HR9 for Pseudomonas putida KT2440 paaF
Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex.
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E. coli PaaF and PaaG form a stable hydratase-isomerase complex.
"stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both"
OpenScientist deep research for PSEPK paaF
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The report supports the crotonase-family enoyl-CoA hydratase assignment.
"encodes a **cytoplasmic enoyl-CoA hydratase** of the **crotonase superfamily** (EC 4.2.1.17)"
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The report assigns hydration of the lower-pathway dicarboxylic enoyl-CoA intermediate.
"syn-hydration of the C2=C3 double bond of 2,3-dehydroadipyl-CoA to yield 3-hydroxyadipyl-CoA"
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Direct kinetic evidence for exact KT2440 Q88HR9 remains unavailable.
"Catalytic parameters (kcat, Km), the exact physiological substrate, and the degree of substrate promiscuity of Q88HR9 have not, to our knowledge, been measured directly."