Connexin-30 supplies the subunits and conducting structure of gap junctions. One core combines channel activity with direct intercellular signaling, transport and structural assembly. The canonical human O95452 sequence is 261 residues; no alternative product is declared. Noncoding-exon usage, mutant constructs and fluorescent fusions are not additional natural proteins (UniProtKB:O95452, PMID:15792634(https://pubmed.ncbi.nlm.nih.gov/15792634/)).
Source-specific positive observations and three short excerpts are collected in the review findings. The source guide distinguishes authentic abstracts and selected original material from full-article access. Cache availability is separate metadata.
The four PAINT assertions were checked against actual ancestral placements, donor records and selected alignment windows. Three follow AN0/PTN008334405, while intercellular transport follows AN365/PTN002633521. Target self-evidence is legitimate; donor count does not replace evaluation of the clade and target architecture. The evidence archive preserves these records and all 35 original contexts.
Ten positive HuRI interactions remain recorded despite generic-binding removal for lack of an informative GJB6 function. Partner class alone does not establish such a function. Full historical clone-sequence equivalence was not established; the literal source identifiers remain available for later checking (PMID:32296183(https://pubmed.ncbi.nlm.nih.gov/32296183/)).
Six unresolved annotations preserve the distinction between evidence that is incomplete and a function that is disproven. Cytoskeletal binding specificity, actin-filament residence, sinoatrial development and hearing require source-specific adjudication. The contextual astroglial barrier annotation remains non-core. No source defect or NEW annotation is asserted.
Clouston syndrome and the historical recessive-hearing disease-validity question are separate. Genetic association, expression and a large genomic deletion do not on their own identify which normal protein activity performs a particular physiological step. The clinical bibliography retains this boundary without converting a negative historical variant screen into a negative biological function.
Reassessed all three substantive requests on PR #6024. The review now weighs the broader hearing evidence, retains two supported cytoskeletal interactions as non-core, and adds tissue and channel-partnership context. Four uncertain rows remain. The source guide records access boundaries and the archive retains the preceding review state. No NEW annotation or natural product was added.