Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Mutant mitochondrial thymidine kinase in mitochondrial DNA depletion myopathy.
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TK2 phosphorylates the pyrimidine deoxyribonucleosides thymidine, deoxycytidine and deoxyuridine; in non-replicating cells mtDNA synthesis depends solely on the mitochondrial salvage kinases TK2 and DGUOK. TK2 mutations reduce muscle mitochondrial TK2 activity to 14-45% of control and cause mtDNA depletion myopathy.
"TK2 phosphorylates deoxythymidine, deoxycytidine and deoxyuridine."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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Proteome-scale AP-MS interactome (BioPlex) reporting a physical interaction of TK2 with GSTK1 (Q9Y2Q3); the basis for a bare protein-binding IPI with no functional specificity for TK2.
"In tandem, these cell-specific interaction networks begin to reveal how interactomes vary with cellular state."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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Defined the high-confidence human mitochondrial proteome (MitoCoP, >1,100 proteins), which includes TK2, corroborating its mitochondrial localization by high-throughput proteomics.
"defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP)."
Multimodal cell maps as a foundation for structural and functional genomics.
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Multimodal (AP-MS + immunofluorescence) cell map of U2OS cells; source of a second bare protein-binding IPI for the TK2-GSTK1 interaction.
"Here we construct a global map of human subcellular architecture through joint measurement of biophysical interactions and immunofluorescence images for over 5,100 proteins in U2OS osteosarcoma cells."
Cloning of the cDNA and chromosome localization of the gene for human thymidine kinase 2.
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Original cloning of human TK2, establishing it as a deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine and deoxyuridine, ~30% similar to dCK and DGUOK, predominantly expressed in liver, pancreas, muscle and brain.
"Human thymidine kinase 2 (TK2) is a deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine."
Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates.
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Molecular cloning and biochemical characterization of human TK2 (purified mitochondrial thymidine kinase), demonstrating broad pyrimidine substrate specificity and complex kinetics, including phosphorylation of antiviral/cytostatic nucleoside analogues.
"The results demonstrate a broad substrate specificity and complex kinetics"
deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2]
UniProtKB O00142 (KITM_HUMAN) TK2 record
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TK2 phosphorylates thymidine, deoxycytidine and deoxyuridine in the mitochondrial matrix using ATP; in non-replicating cells mtDNA synthesis depends solely on TK2 and DGUOK. Has a cleaved N-terminal mitochondrial transit peptide (1-33) and belongs to the DCK/DGK family. Deficiency causes MTDPS2 and PEOB3.
"Phosphorylates thymidine, deoxycytidine, and deoxyuridine in"