Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
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The study generated a human E2/E3-RING interaction network using yeast two-hybrid screening.
"In this study, yeast two-hybrid (Y2H) screens were combined with true homology modeling methods to generate a high-density map of human E2/E3-RING interactions."
A proteome-scale map of the human interactome network.
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A binary interaction does not by itself establish physiological relevance.
"Even with nearly complete, high-quality reference interactome maps of biophysical interactions, how can the biological relevance of each interaction be evaluated under physiological conditions?"
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
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The study evaluates variants through interaction perturbation rather than all aspects of protein function.
"Though there are many ways by which a missense mutation can impact a protein’s function, such as by destabilizing protein folding, we evaluate the disruptiveness of a mutation within our framework exclusively on its capacity to disrupt protein interactions, measured experimentally or through prediction."
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
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The study profiled missense-variant effects across a large pre-existing interaction set.
"To address this gap, we leverage the ExAC database of 60,706 human exomes to investigate experimentally the impact of 2009 missense single nucleotide variants (SNVs) across 2185 protein-protein interactions, generating interaction profiles for 4797 SNV-interaction pairs, of which 421 SNVs segregate at > 1% allele frequency in human populations."
A reference map of the human binary protein interactome.
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HuRI is a systematic binary-interaction map rather than an endogenous context-specific interaction study.
"It remains infeasible to assemble a reference interactome map by systematically identifying endogenous PPIs in thousands of physiological and pathological cellular contexts11,12."
Transcriptomic characterization of Lonrf1 at the single-cell level under pathophysiological conditions.
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The only LONRF1-focused study reports expression patterns but leaves its physiological function unresolved.
"However, the physiological implications of other LONRF isozymes remain unclear."
LONRF2 is a protein quality control ubiquitin ligase whose deficiency causes late-onset neurological deficits.
Regulation of inorganic polyphosphate is required for proper vacuolar proteolysis in fission yeast.
UniProt record for human LONRF1 (Q17RB8)
Reproducible LONRF1 domain and motif analysis
Transfer of Ub from E2 to substrate and release of E2
Release of E3 from polyubiquitinated substrate
Polyubiquitination of substrate
Interaction of E3 with substrate and E2-Ub complex