fliG (PP_4368, Q88ET5) notes

2026-11-03 re-review

• Deep research: just deep-research PSEPK fliG --provider perplexity failed (provider 'perplexity' not available); just mod-description-deep-research skipped (no MOD provider configured for PSEPK). Existing falcon report used; every falcon quote was checked as a verbatim substring.
• UniProt quotes now cite the local flat file (file:PSEPK/fliG/fliG-uniprot.txt). ARBA-only, imported (ECO:0000313), DE-name and DR GO lines are not used as support.
• Most rows lacked supported_by; added quotes from PMID:31452860 (full text) PMID:31452860 PMID:31452860, the UniProt PIRNR-backed FUNCTION line, NCBIfam TIGR00207 and the falcon fliEFG operon statement.
• Location rows: GO:0009425 basal body and GO:0009288 flagellum MODIFY -> GO:0009433 C ring (correct but general); GO:0005886 MODIFY -> GO:0009898 cytoplasmic side of plasma membrane (peripheral, PIRNR-backed location). GO:0003774 MODIFY -> GO:0140605 (contributes_to). Chemotaxis KEEP_AS_NON_CORE (FliG performs the switching step but its core role is rotation). GO:0071973 ACCEPT.
• Added NEW GO:0005198 structural molecule activity (IC) as a second core function: the C-ring module asserts this complex-level activity and FliG forms the ring scaffold. Status remains COMPLETE.

2026-11-06 comparative flagellar audit

Reviewed every existing annotation and the biological summary/core functions. Original annotation payloads, including historical NEW proposals, are preserved. Family/domain evidence supports conserved structural assignments; comparative Salmonella or other bacterial assays are not presented as KT2440 experiments. Exact subunit counts and interaction geometries are not transferred as target measurements.

GO:0003774 explicitly includes electrochemical-gradient-driven flagellar rotation. Rotor/switch structural roles are distinguished from autonomous torque generation. The C-ring/MS-ring CC terms were verified against ontology ancestry and remain valid substructure locations.

2026-11-06 — PR6138 evidence follow-up

Restored explicit CheY-P-to-FliG switching and rotor–stator interface evidence from PMID31452860. Comparative residue labels remain attributed to that source. Current GO0140605 descends from GO0003774; the older wrong-kind-of-motor wording would be incorrect. Removed unused PMID39766194 reference.