Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:16030218

Characterization of a multigene-encoded sodium/hydrogen antiporter (sha) from Pseudomonas aeruginosa: its involvement in pathogenesis.

The PA1054-PA1059 cluster encodes a Na+/H+ antiporter; the abstract reports mutation of PA1054 only.

"We disrupted PA1054 (a shaA homologue gene) and determined its effect on Na+ tolerance during growth, Na+ efflux, and pathogenicity in mice."

PMID:11004162

Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.

B. subtilis MrpF alone restores cholate transport and increases Na+ efflux in an mrp null strain.

"Expression of mrpF in the mrp null (VKN1) strain also restored cholate transport and increased Na+ efflux, indicating that MrpF does not require even low levels of other mrp gene expression for its own function."

PMID:20624916

Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4 that affect Na+/H+ antiport activity, sodium exclusion, individual Mrp protein levels, or Mrp complex formation.

Mrp antiport requires the hetero-oligomeric complex of all Mrp subunits.

"Antiport activity requires hetero-oligomeric complexes of all six or seven hydrophobic Mrp proteins (MrpA-MrpG)."

PMID:29218041

Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea.

MrpF-D32A abolishes Mrp antiport and complex formation.

"In category 1, MrpD-D75A, MrpD-R258A, MrpE-T113Y, and MrpF-D32A were studied, and their Na+/H+ antiport activity was found to be completely lost, with no Mrp complex detected."

PMID:29754813

Structure of an Ancient Respiratory System.

MrpF corresponds to MBH subunit B; its conserved Asp is needed for antiport.

"Mutations D32A and R33A in MrpF, equivalent to D29 and R30 of MBH subunit B, either abolished (D32A) or reduced (R33A) the H+/Na+ antiporter activity"

file:PSEAE/PA1058/PA1058-uniprot.txt

UniProt entry Q9I4R6 (Q9I4R6_PSEAE)

file:PSEAE/PA1058/PA1058-bioinformatics/RESULTS.md

Composition of PAINT node PTN002162906 (PTHR34702) and its leaf IBAs