Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
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For lacS the IBA source is PAINT node PTN008498319 (PTHR10353), whose galactosyltransferase IBD is seeded only by plant SFR2 and which also carries a negated beta-glucosidase IRD. lacS is placed in subfamily SF209, which is named after SFR2, together with other archaeal GH1 beta-galactosidases.
Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene.
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lacS encodes a 489-aa beta-galactosidase unrelated in sequence to E. coli lacZ.
"There appears to be no evident similarity between this and the Escherichia coli lacZ sequence, thus suggesting that the two enzymes have analogous function, but are not homologous."
Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties.
Evidence that beta-Galactosidase of Sulfolobus solfataricus Is Only One of Several Activities of a Thermostable beta-d-Glycosidase.
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beta-glucosides and beta-fucosides are preferred over beta-galactosides, at a common active site.
"beta-glucosides and beta-fucosides represent the preferred substrates"
Exo-glucosidase activity and substrate specificity of the beta-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus.
Identification of two glutamic acid residues essential for catalysis in the beta-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus.
Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.
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Retaining mechanism with Glu387 nucleophile and Glu206 acid/base.
"Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst"
Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability.
Characterization and functional complementation of a nonlethal deletion in the chromosome of a beta-glycosidase mutant of Sulfolobus solfataricus.
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lacS plus the adjacent putative transporter lacTr are required for growth on lactose.
"The presence of both lacS and lacTr was necessary to sustain growth on the specific sugar and hence to complement the mutation"
Coordinate transcriptional control in the hyperthermophilic archaeon Sulfolobus solfataricus.
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lacS expression is transcriptionally regulated by carbon source quality together with other glycosyl hydrolases.
"acts at the level of transcription to coordinate synthesis of three physically unlinked glycosyl hydrolases implicated in carbohydrate utilization"
Extragenic pleiotropic mutations that repress glycosyl hydrolase expression in the hyperthermophilic archaeon Sulfolobus solfataricus.
The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfolobus solfataricus.
Tailoring the substrate specificity of the beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus.
Thermal stability and aggregation of sulfolobus solfataricus beta-glycosidase are dependent upon the N-epsilon-methylation of specific lysyl residues: critical role of in vivo post-translational modifications.
Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase.
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LacS from strain P2 produces galacto-oligosaccharides from lactose by transglycosylation in vitro.
"The enzymatic properties and optimal condition for transglycosylation reaction of the enzymes were investigated in detail."
Structural determinants allowing transferase activity in SENSITIVE TO FREEZING 2, classified as a family I glycosyl hydrolase.
Freezing tolerance in plants requires lipid remodeling at the outer chloroplast membrane.