Annotation inferences using phylogenetic trees

BUB3.1 functions are phylogenetically conserved across eukaryotes

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Keywords mapped to GO terms, some may be over-generalized

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Subcellular locations consistently mapped

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

GO_REF:0000122

AtSubP analysis

Computational prediction of nuclear localization confirmed experimentally

PMID:18223036

Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases.

Identified DWD proteins in Arabidopsis that may serve as CUL4 substrate receptors
WD40-containing proteins with DWD motif can interact with DDB1

PMID:18508582

The Arabidopsis checkpoint protein Bub3.1 is essential for gametophyte development.

BUB3.1 is essential for male and female gametophyte development
Homozygous knockouts are lethal

PMID:19710914

Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division.

BUB3.1 interacts physically with BUBR1 and MAD2
BUB3.1 localizes to unattached kinetochores upon SAC activation
BUB3.1 displays cell cycle-regulated expression with G2/M peak
BUB3.1 unexpectedly localizes to phragmoplast midline during cytokinesis
BUB3.1 associates with both kinetochores and spindle microtubules during metaphase arrest

PMID:20706207

Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.

High-throughput TAP-MS identified BUB3.1 interactions in cell cycle network
BUB3.1 connected to mitotic checkpoint protein network

PMID:21687678

Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

BUB3.1 interacts with CDC20-1 and CDC20-2
AtCDC20.1 and AtCDC20.2 are components of MCC with BUB3.1

DOI:10.1038/s41477-018-0192-z

Role of the BUB3 protein in phragmoplast microtubule reorganization during cytokinesis

BUB3.1 and BUB3.2 interact with MAP65-3
BUB3.1/BUB3.2 promote phragmoplast microtubule reorganization
bub3.1 bub3.2 double mutants show cytokinesis defects

DOI:10.1073/pnas.2316583121

A coadapted KNL1 and spindle assembly checkpoint axis orchestrates precise mitosis in Arabidopsis

BUB3.3 (not BUB3.1) is recruited to kinetochores by KNL1
Paralog specialization in Arabidopsis BUB3 family

DOI:10.1073/pnas.2322677121

The <i>Arabidopsis</i> BUB1/MAD3 family protein BMF3 requires BUB3.3 to recruit CDC20 to kinetochores in spindle assembly checkpoint signaling

BUB3.3 mediates CDC20 recruitment to kinetochores via BMF3
Functional distinction between BUB3 paralogs in plants

file:ARATH/BUB3.1/BUB3.1-deep-research-falcon.md

Deep research summary for BUB3.1

BUB3.1 is a WD40-repeat scaffold/adaptor involved in spindle checkpoint complexes and plant-specific phragmoplast cytokinesis.
Recent family-level work clarifies that BUB3.3 handles specialized kinetochore recruitment while BUB3.1/BUB3.2 have prominent phragmoplast and MAP65-3-associated roles.