Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs
Combined Automated Annotation using Multiple IEA Methods
Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.
-
ZDHHC23 (with ZDHHC22) controls palmitoylation of the BK channel S0-S1 loop and promotes its surface expression; tagged zDHHC23 localizes mainly to Golgi/TGN. Experiments were in HEK293 cells.
"palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23."
S-Acylation controls functional coupling of BK channel pore-forming α-subunits and β1-subunits.
-
Global Zdhhc23 knockout (Zdhhc23tm1(KOMP)Vlcg) reduces endogenous BK alpha-subunit S-acylation by ~60% in mouse aortic smooth muscle cells and reduces BK current, without changing alpha-subunit surface expression.
"Acyl-RAC performed on VSMCs from Zdhhc23−/− mice displayed a significant (∼60%) reduction in BK channel α-subunit S-acylation (Fig. 5A)."
GFAP palmitoylcation mediated by ZDHHC23 in spinal astrocytes contributes to the development of neuropathic pain.
-
Zdhhc23 knockdown in the mouse astrocyte line MA-C reduces GFAP palmitoylation; Zdhhc23 is upregulated in spinal dorsal horn in a mouse cancer-pain model.
"Further studies showed that GFAP significantly interacts with the palmitoyltransferase ZDHHC23 in astrocytes and that ZDHHC23 expression knockdown significantly downregulated GFAP palmitoyl modification levels."
Identification of PSD-95 palmitoylating enzymes.
UniProt record for mouse Zdhhc23