Gene Ontology annotation through association of InterPro records with GO terms
A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes.
-
Defines the anSME family from a Firmicutes enzyme that converts the critical cysteine of a sulfatase to formylglycine under anaerobic conditions.
"we have also identified a new Cys-type sulfatase-maturating enzyme that catalyzes the conversion of cysteine into FGly, in anaerobic conditions"
First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants.
-
E. coli K-12 matures heterologous Cys-type sulfatases only aerobically, and deleting aslB, ydeM and yidF does not impair that maturation.
"knocking-out of aslB, ydeM and yidF does not impair Cys-type sulfatase maturation"
Anaerobic sulfatase maturase AslB from Escherichia coli activates human recombinant iduronate-2-sulfate sulfatase (IDS) and N-acetylgalactosamine-6-sulfate sulfatase (GALNS).
-
Co-expression of AslB in E. coli BL21(DE3) increased the activity of co-expressed human sulfatases IDS (4.6-fold) and GALNS (1.5 to 2-fold).
"IDS activity was increased in 4.6-fold when co-expressed with AslB"
Post-translational formylglycine modification of bacterial sulfatases by the radical S-adenosylmethionine protein AtsB.
-
Klebsiella AtsB, the closest characterised homolog of AslB, needs SAM and all three of its FeS centres to convert sulfatase serine to formylglycine.
"SAM is the critical co-factor for formation of a functional AtsB.SAM.sulfatase complex and for FGly formation by AtsB"
UniProtKB entry P25550 (ASLB_ECOLI)
AslB and YdeM vs characterised anaerobic sulfatase-maturating enzymes
-
AslB keeps all eleven [4Fe-4S] cluster cysteine ligands and the catalytic aspartate of the C. perfringens anSME, and is 44.7% identical to Klebsiella AtsB.
"AslB keeps 16 of 17 reference features"