Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Comparison of the rat nucleolar protein nopp140 with its yeast homolog SRP40. Differential phosphorylation in vertebrates and yeast.
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SRP40 encodes an acidic, serine-rich 41 kDa protein whose C-terminus is 59% identical to that of rat Nopp140; it localizes to the yeast nucleolus, is phosphorylated by casein kinase II (to a lesser extent than Nopp140), and is required at a specific cellular concentration for optimal growth (both overexpression and deletion are deleterious).
"SRP40 localizes to the yeast nucleolus and is required at a specific cellular concentration for optimal growth as indicated by the negative effect on cell growth of both overexpression and deletion of its gene."
Genetic interaction between a chaperone of small nucleolar ribonucleoprotein particles and cytosolic serine hydroxymethyltransferase.
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Srp40p is a nonessential nucleolar protein proposed to chaperone the >100 snoRNPs required for rRNA maturation; its genetic depletion specifically depletes box H/ACA snoRNAs, and rat Nopp140 restores growth and box H/ACA snoRNA stability, confirming it as the functional yeast homolog of Nopp140.
"Srp40p is a nonessential yeast nucleolar protein proposed to function as a chaperone for over 100 small nucleolar ribonucleoprotein particles that are required for rRNA maturation."
Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli.
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The human Nopp140 repeat domain uses phosphorylation-dependent, multivalent interactions with IDRs in snoRNP core proteins and RNA polymerase I-associated PAF49 to form a dense fibrillar component condensate that promotes rRNA modification.
"Here we demonstrate that Nopp140 concentrates intrinsically disordered and nuclear localization signal (NLS)-rich protein regions (IDRs), including a newly identified RNA polymerase I C-terminal domain (CTD) of the RNA polymerase I-associated factor PAF49."
Manipulating the nucleolar serine-rich protein Srp40p in Saccharomyces cerevisiae may improve isobutanol production
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Overexpressing srp40 in engineered W303-1A derivatives increased isobutanol production and tolerance, but the abstract reports a strain-engineering phenotype and transcriptome changes rather than a direct native molecular activity for Srp40p.
"In summary, the overexpression of srp40 could increase isobutanol production and tolerance in S. cerevisiae."
Interactions between three common subunits of yeast RNA polymerases I and III.
SGD gene description for SRP40 (yeast)
UniProt entry P32583 (SRP40)