TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Terminal-primer identity analysis of native KT2440 constructs in PMID:40236303
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Published terminal primers distinguish the named native constructs; this does not verify the full cloned CDS or measure enzymatic activity.
"The thrB_ecoRI_F primer matches MSVFTPVT at the N terminus of ThrB/PP_0121/Q88RK8, and thrB_not_R matches its terminal LHLPFAL."
Synthetic pathways for microbial biosynthesis of valuable pyrazine derivatives using genetically modified Pseudomonas putida KT2440.
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Native constructs were overexpressed in KT2440; terminal primer mapping identifies the corresponding locus. Joint engineering and soluble expression do not establish individual kinetics, necessity or feedback inhibition.
"the remaining genes of the metabolic pathway – thrB and thrC – were overexpressed as well."
UniProtKB record for Q88RK8 (thrB)
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Family, domain or curated catalytic-feature support; evidence remains an inference unless specifically stated as measured.
"Reaction=L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)"
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Family, domain or curated catalytic-feature support; evidence remains an inference unless specifically stated as measured.
"HAMAP; MF_00301; Homoser_kinase_2; 1."
Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.
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Review of the branched aspartate-family network; target assignments depend on independent target family evidence and, where available, native primary cloning.
"A multibranched web of biochemical pathways of central importance is initiated by the action of aspartokinase (Ask) in a reaction that combines aspartate and ATP."
Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes.
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Comparative P. aeruginosa study of threonine genes, not direct KT2440 ThrB genetics.
"Three genes from Pseudomonas aeruginosa involved in threonine biosynthesis, hom, thrB and thrC, encoding homoserine dehydrogenase (HDH), homoserine kinase (HK) and threonine synthase (TS), respectively, have been cloned and sequenced."
Existing openscientist research report for PSEPK thrB