Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Divergent hTAFII31-binding motifs hidden in activation domains.
A second calcineurin binding site on the NFAT regulatory domain.
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Identification of a second calcineurin-binding site (LxVP-type) in the NFATc regulatory domain.
"Here we report the identification of a second Cn-binding element in NFATc, which synergizes with the previously identified element."
Targeted disruption of NFATc3, but not NFATc4, reveals an intrinsic defect in calcineurin-mediated cardiac hypertrophic growth.
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Nfatc1 disruption causes embryonic lethality from aberrant heart valve formation (cited background in an NFATc3 knockout study).
"Disruption of the NFATc1 gene resulted in embryonic lethality due to aberrant heart valve formation and cardiac insufficiency"
Regulation of apoptosis by the Ft1 protein, a new modulator of protein kinase B/Akt.
Co-operative interactions between NFAT (nuclear factor of activated T cells) c1 and the zinc finger transcription factors Sp1/Sp3 and Egr-1 regulate MT1-MMP (membrane type 1 matrix metalloproteinase) transcription by glomerular mesangial cells.
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NFATc1 binds and transactivates the MT1-MMP promoter in mesangial cells, cooperating with Sp1 and Egr-1; blocked by cyclosporin A.
"Transfection with an NFAT c1 expression plasmid, or activation of calcineurin with a calcium ionophore, yielded major increases in NFAT c1 nuclear DNA-binding activity, MT1-MMP transcription and protein synthesis, which were additive with the lower levels of transactivation provided by the proximal Sp1 and the overlapping Sp1/Egr-1 sites."
Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1 and PU.1.
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RANKL-induced NFATc1 accumulates in osteoclast nuclei and complexes with PU.1 on the cathepsin K promoter.
"RANKL-induced NFATc1 forms a complex with PU.1 in nuclei of osteoclasts following the nuclear accumulation of NFATc1 phosphorylated by the activated p38 MAP kinase."
A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT.
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Phosphorylated NFAT resides in the cytoplasm; calcineurin dephosphorylation drives nuclear import, opposed by DYRK, GSK3 and CK1.
"In resting cells, NFAT proteins are heavily phosphorylated and reside in the cytoplasm; in cells exposed to stimuli that raise intracellular free Ca2+ levels, they are dephosphorylated by the calmodulin-dependent phosphatase calcineurin and translocate to the nucleus."
Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation.
NFAT binding and regulation of T cell activation by the cytoplasmic scaffolding Homer proteins.
Nuclear factor of activated T cells (NFAT) proteins repress canonical Wnt signaling via its interaction with Dishevelled (Dvl) protein and participate in regulating neural progenitor cell proliferation and differentiation.
The molecular mechanism of substrate engagement and immunosuppressant inhibition of calcineurin.
MicroRNA-124 suppresses the transactivation of nuclear factor of activated T cells by targeting multiple genes and inhibits the proliferation of pulmonary artery smooth muscle cells.
The LxVP and PxIxIT NFAT motifs bind jointly to overlapping epitopes on calcineurin's catalytic domain distant to the regulatory domain.
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The NFATc1 LxVP peptide binds the calcineurin catalytic subunit at an epitope overlapping the PxIxIT site.
"Here, we present evidence that a 15-mer peptide from NFATc1 containing the conserved LxVP sequence binds to a CnA region overlapping with the PxIxIT-binding epitope."
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
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TAP/MS: NFATC1 complexes with JUN, CREB1 and ATF1/2/3 only in the chromatin fraction.
"Western analysis confirmed that NFATC1 binds to JUN, CREB1 and ATF1/2 predominantly in the chromatin fraction"
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
CD69-oxLDL ligand engagement induces Programmed Cell Death 1 (PD-1) expression in human CD4 + T lymphocytes.
NF-AT components define a family of transcription factors targeted in T-cell activation.
Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc.
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Characterization of the NFATc.beta isoform; its IL-2 reporter activity is CsA-sensitive (does not show FK506 binding by NFATC1).
"Transient expression of NFATc.beta was capable of activating an interleukin-2 NFAT-driven reporter gene in stimulated Jurkat cells in a CsA-sensitive manner."
Solution structure of the core NFATC1/DNA complex.
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Solution structure of the human NFATC1 Rel homology DNA-binding domain bound to the IL2 promoter ARRE2 site.
"Here we report the solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the ARRE2 DNA site from the interleukin-2 promoter."
Calcineurin binds NFATC1,2,3
Calcineurin binds and dephosphorylates NFAT
Translocation of CaN:CaM:NFAT to nucleus
Calcineurin binds and dephosphorylates NFAT1 in response to WNT/Ca2+ signaling
Translocation of NFATC1:CaN:CaM to nucleus
Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts.
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NFATc1 is induced by RANKL and is necessary and sufficient for osteoclast terminal differentiation (mouse).
"NFATc1-deficient embryonic stem cells fail to differentiate into osteoclasts in response to RANKL stimulation, and that ectopic expression of NFATc1 causes precursor cells to undergo efficient differentiation without RANKL signaling"
Multiple NF-ATc isoforms with individual transcriptional properties are synthesized in T lymphocytes.
Falcon deep research report for human NFATC1
Non-redundant functions of NFATc1 in survival and NFATc2 in generation of exhausted CD8(+) T cells.
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Chronic antigen receptor stimulation induces the short NFATc1/alphaA isoform, which promotes survival of exhausted CD8+ T cells, whereas NFATc2 drives terminal exhaustion (mouse).
"Chronic antigen receptor stimulation induces the expression of NFATc1/αA, a short isoform of NFATc1 that promotes TEX cell survival."
NFAT signaling is indispensable for persistent memory responses of MCMV-specific CD8+ T cells.
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NFATc1 ablation impairs inflation of MCMV-specific CD8+ memory T cells, with strongest effects when NFATc2 is also deleted (mouse).
"NFATc1 ablation elicited robust phenotypes in isolation, but the strongest effects were observed when both NFAT genes were missing."
NFATc1 in CD4(+) T cells and CD11c(+) dendritic cells drives T(H)2-mediated eosinophilic inflammation in allergic asthma.
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Conditional Nfatc1 deletion in CD4+ T cells impairs TH2 polarization; deletion in CD11c+ cells impairs DC maturation and T cell priming (mouse).
"Nfatc1's absence in CD4+ T cells directly hampered TH2 cell polarization and functionality"
Calcineurin/NFATc1 pathway represses cellular cytotoxicity by modulating histone H3 expression.
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In human MCF7 cells NFATc1 knockdown raises histone H3 mRNAs, and NFATc1 occupies histone gene promoters (ChIP-qPCR; reanalysed HUVEC ChIP-seq), suggesting calcium/calcineurin-dependent repression of histone transcription.
"NFATc1 binds to the promoter regions of many histone genes and that histone transcription is downregulated in a manner dependent on intracellular calcium levels."
Chromatin-Independent Interplay of NFATc1 and EZH2 in Pancreatic Cancer.
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NFATc1 activates Ezh2 transcription and forms a complex with EZH2 in PDAC, apparently independent of joint gene regulation.
"NFATc1 targets the Ezh2 gene for transcriptional activation and biochemically interacts with the methyltransferase in murine and human PDAC."