Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway.
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E1beta2 is assigned to the muscle-enriched E1 pair proposed to function as a mitochondrial acetaldehyde-producing decarboxylase. The study combines transcript stoichiometry, E1 protein/localization observations and structural modeling, not purified-paralog reconstitution.
"Together, these data suggest that the protein products of the E1α3 and E1β2 paralogs combine to produce a specific E1 enzyme"
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Mitochondrial targeting and E1 localization support compartment assignment; antibody observations are not beta2-specific.
"The presence of 5′ mitochondrial import signals in both Carassius PDC subunits supports a mitochondrial localization"
UniProtKB CARAX A0A288DUG2 source record
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The record links this accession to the submitted E1beta2 sequence, as independently verified in the ENA record description.
"DR EMBL; KF960828; AHG05820.1; -; mRNA."