Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The layered structure of human mitochondrial DNA nucleoids.
Proteomic analysis of increased Parkin expression and its interactants provides evidence for a role in modulation of mitochondrial function.
Structural and biochemical characterization of human mitochondrial branched-chain α-ketoacid dehydrogenase phosphatase.
Architecture of the human interactome defines protein communities and disease networks.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Purification and characterization of human liver branched-chain alpha-keto acid dehydrogenase complex.
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The purified human liver BCKDH complex oxidizes the branched-chain keto acids KIV, KIC, and KMV (from valine, leucine, isoleucine) with Km 14-17 microM, and absolutely requires NAD and CoASH.
"The BCKADH effectively oxidized all of KIV, KIC, and KMV, yielding apparent Km values in the range of 14-17 microM for those alpha-keto acids."
Multimodal cell maps as a foundation for structural and functional genomics.
BCKDK phosphorylates BCKDH
PPM1K dephosphorylates p-BCKDH
LIPT1 transfers lipoyl group from lipoyl-GCSH to DBT/DLST
Branched-chain amino acid catabolism
LONP1 degrades mitochondrial matrix proteins
LONP1 binds mitochondrial matrix proteins
BCKDHA:BCKDHB tetramer decarboxylates KIC, KMVA, KIV
DBT transfers BCAA to CoA
DLD dimer dehydrogenates dihydrolipoyl
DBT loss-of-function mutants don't synthesize BCAA-CoA
BCKDHA or BCKDHB loss-of-function mutants don't synthesize BCAA-CoA
Loss-of-function DLD mutants don't dehydrogenate dihydrolipoyl DBT
BCKDK loss-of-function mutations do not phosphorylate BCKDH
H139Hfs13* PPM1K does not dephosphorylate BCKDH