Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
EPS8 and E3B1 transduce signals from Ras to Rac.
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EPS8, ABI1 (E3B1), and SOS1 assemble into a trimeric complex exhibiting Rac-specific GEF activity downstream of Ras.
"We also show that Eps8, E3b1 and Sos-1 form a tri-complex in vivo that exhibits Rac-specific GEF activity in vitro."
Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system.
Isolation of hNap1BP which interacts with human Nap1 (NCKAP1) whose expression is down-regulated in Alzheimer's disease.
The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia.
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ABI1 and ABI2 localize specifically to sites of active actin polymerization at the tips of lamellipodia and filopodia.
"We show that Abi-1 and Abi-2b fused to enhanced yellow fluorescent protein (EYFP) are recruited to the tips of lamellipodia and filopodia."
Phosphoinositide 3-kinase activates Rac by entering in a complex with Eps8, Abi1, and Sos-1.
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ABI1 recruits PI3K via p85 into the EPS8-ABI1-SOS1 complex to activate Rac.
"Here, we show that Abi1 (also known as E3b1) recruits PI3K, via p85, into a multimolecular signaling complex that includes Eps8 and Sos-1."
Abl interactor 1 promotes tyrosine 296 phosphorylation of mammalian enabled (Mena) by c-Abl kinase.
Induction of colonic epithelial cell apoptosis by p47-dependent oxidants.
The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway.
Abi1 is essential for the formation and activation of a WAVE2 signalling complex.
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ABI1 binds the WHD of WAVE2, assembles the WAVE2-ABI1-NAP1-PIR121 complex, and is essential for Rac-dependent lamellipodia protrusion.
"Abi1 interacts directly with the WHD domain of WAVE2, increases WAVE2 actin polymerization activity and mediates the assembly of a WAVE2-Abi1-Nap1-PIR121 complex."
Abl interactor 1 (Abi-1) wave-binding and SNARE domains regulate its nucleocytoplasmic shuttling, lamellipodium localization, and wave-1 levels.
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ABI1 binds WAVE1 via its N-terminal WAB domain for lamellipodium tip targeting and undergoes leptomycin B-sensitive nucleocytoplasmic shuttling.
"Here, we show that Abi-1 binds the actin nucleator protein Wave-1 through an amino-terminal Wave-binding (WAB) domain and that disruption of the Abi-1-Wave-1 interaction prevents Abi-1 from reaching the tip of the lamellipodium."
IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.
ABI2-deficient mice exhibit defective cell migration, aberrant dendritic spine morphogenesis, and deficits in learning and memory.
Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes.
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ABI1 binds N-WASP with nanomolar affinity and cooperates with Cdc42 to stimulate N-WASP-dependent endocytosis and vesicular transport distinct from WAVE-mediated protrusion.
"Consistently, Abi1 binds to N-WASP with nanomolar affinity and, cooperating with Cdc42, potently induces N-WASP activity in vitro."
Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis.
Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching.
NESH (Abi-3) is present in the Abi/WAVE complex but does not promote c-Abl-mediated phosphorylation.
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ABI1 and ABI2, unlike ABI3/NESH, bind c-Abl and promote c-Abl-mediated phosphorylation of WAVE2 and Mena within the WAVE complex.
"Although Abi-2, like Abi-1, promoted the c-Abl-mediated phosphorylation of Mena and WAVE2, NESH (Abi-3) had no such effect."
Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation.
Allosteric inhibition of the nonMyristoylated c-Abl tyrosine kinase by phosphopeptides derived from Abi1/Hssh3bp1.
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Phosphorylated Tyr213 and a proximal PXXP motif of ABI1 concurrently engage the c-Abl SH2 and SH3 domains to mediate Y213-dependent allosteric inhibition of nonmyristoylated c-Abl in trans, reducing pY412 phosphorylation and pervanadate-induced c-Abl kinase activity upon coexpression of wild-type ABI1 in LNCaP cells.
"The mechanism, which is pertinent to the nonmyristoylated c-Abl kinase, involves high affinity concurrent binding of the phosphotyrosine pY213 to the Abl SH2 domain and binding of a proximal PXXP motif to the Abl SH3 domain."
Large-scale proteomics and phosphoproteomics of urinary exosomes.
The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1.
Neutrophils establish rapid and robust WAVE complex polarity in an actin-dependent fashion.
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Live-cell TIRF microscopy tracks fluorescently tagged Abi1 (Abi1-YFP) with the WAVE complex at the tips of protruding lamellipodia at the leading edge of migrating B16F10 murine cells.
"A B16F10 fibroblast cell expressing Abi1-YFP was imaged at 37°C by TIRF while migrating on fibronectin. The playback speed is 10 frames/s. This movie corresponds to Figure 1A."
Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during Caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis.
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Human ABI1 binds directly to the immobilized SH3 domain of TOCA-1 in vitro and co-immunoprecipitates with endogenous and ectopically expressed TOCA-1.
"To this end, we utilized the SH3 domain of TOCA-1 to search for novel interactors, employing phage display libraries of human polypeptides [47]. We found ABI-1 among the most represented interactors (not shown). We validated this interaction by showing that endogenous and ectopically expressed TOCA-1 and ABI-1 coimmunoprecipitated (Figure 7A and 7B), and interacted in in vitro binding experiments using immobilized SH3 domain of TOCA-1 (Figure S7C)."
Abi1/Hssh3bp1 pY213 links Abl kinase signaling to p85 regulatory subunit of PI-3 kinase in regulation of macropinocytosis in LNCaP cells.
Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Structure and control of the actin regulatory WAVE complex.
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Crystal structure of the heteropentameric WAVE regulatory complex defines the WAVE1:Abi2:HSPC300 trimer and Sra1:Nap1 dimer subcomplexes.
"MiniWRC can be delineated into two subcomplexes: an Sra1:Nap1 dimer and a WAVE1:Abi2:HSPC300 trimer"
Essential role for Abi1 in embryonic survival and WAVE2 complex integrity.
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Abi1 knockout in mice destabilizes WAVE1, WAVE2, NAP1, and Sra-1/PIR121 and causes embryonic lethality at ~E11.5.
"Western blotting analysis of Abi1-KO cell lysates indicated reduced levels of the WAVE complex components WAVE1 and WAVE2, Nap1, and Sra-1/PIR121."
Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses.
CDK1-mediated phosphorylation of Abi1 attenuates Bcr-Abl-induced F-actin assembly and tyrosine phosphorylation of WAVE complex during mitosis.
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CDK1/cyclin B phosphorylates ABI1 at Ser216 at mitotic entry to attenuate WAVE complex tyrosine phosphorylation and F-actin assembly.
"We identified serine 216 of Abi1 as a target of CDK1/cyclin B kinase that is phosphorylated in cells at the onset of mitosis."
Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion.
E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex.
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A linker region between the proline-rich region and C-terminal SH3 domain of ABI1 is essential for c-Abl binding and WAVE2 phosphorylation.
"Structural studies showed that a linker region between the proline-rich regions and the Src homology 3 (SH3) domain of Abi-1 is crucial for its interaction with c-Abl and c-Abl-mediated phosphorylation of WAVE2."
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
PTEN dephosphorylates Abi1 to promote epithelial morphogenesis.
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PTEN dephosphorylates ABI1 at Tyr213 and Ser216, triggering calpain-mediated ABI1 degradation and WRC downregulation during epithelial polarization.
"We demonstrate that PTEN dephosphorylation of Abi1 at Y213 and S216 results in Abi1 degradation through the calpain pathway. This leads to down-regulation of the WRC and reorganization of the actin cytoskeleton."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Coronatine orchestrates ABI1-mediated stomatal opening to facilitate bacterial pathogen infection through importin β protein SAD2.
Multimodal cell maps as a foundation for structural and functional genomics.
Abl-interactor-1, a novel SH3 protein binding to the carboxy-terminal portion of the Abl protein, suppresses v-abl transforming activity.
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Mouse Abl-interactor-1 (Abi-1, identified in a mouse leukemia cell line and mapped to mouse chromosome 2) requires its C-terminal SH3 domain for binding to Abl proteins.
"A novel cellular protein, Abl-interactor-1 (Abi-1), which specifically interacts with the carboxy-terminal region of Abl oncoproteins, has been identified in a mouse leukemia cell line. The protein exhibits sequence similarity to homeotic genes, contains several polyproline stretches, and includes a src homology 3 (SH3) domain at its very carboxyl terminus that is required for binding to Abl proteins. The abi-1 gene has been mapped to mouse chromosome 2 and is genetically closely linked to the c-abl locus."
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Overexpression of mouse abi-1 in NIH-3T3 fibroblasts suppresses p160v-Abl-mediated transformation.
"When overexpressed in NIH-3T3 cells, abi-1 potently suppresses the transforming activity of Abelson leukemia virus expressing the full-length p160v-abl kinase"
Isolation and characterization of e3B1, an eps8 binding protein that regulates cell growth.
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E3B1/ABI1 is a cytosolic phosphoprotein that binds the SH3 domains of EPS8 and Abl and is hyperphosphorylated after EGF stimulation.
"E3B1 binds to the SH3 domain of eps8 and Abl in vitro. We also demonstrated that e3B1 associates with eps8 in vivo."
Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrin-based membrane skeleton.
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Hssh3bp1/ABI1 contains two alpha-I spectrin SH3-binding regions and one Abl SH3-binding region.
"Mapping of SH3 binding regions revealed the presence of two alphaI SH3 domain binding regions and one Abl-SH3 domain binding region."
RAC1:GTP and PIP3 bind WAVE Regulatory Complex (WRC)
(WASPs, WAVE):G-actin:ARP2/3 binds F-actin
p-ERK phosphorylates WAVEs and ABI
RAC1 binds effectors at the plasma membrane
RAC3 binds effectors at the plasma membrane
Affinage mechanistic annotation for ABI1 (human)