Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Natural Variation in the Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast.
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YDR090C was named ILT1 (ionic liquid tolerance 1) because its deletion sensitizes yeast to the imidazolium ionic liquid [C2C1im]Cl; complementation restores tolerance, and the protein also protects against other cationic toxins including crystal violet and [C4C1im]Cl in the BY (S288c) background.
"ILT1 functions in tolerance to a range of cationic toxins in the BY strain background"
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Ilt1-GFP fusion protein localizes to the plasma membrane, consistent with the independent Huh et al. GFP-localization dataset.
"both Sge1PLL-GFP and Ilt1-GFP fusion proteins localized to the plasma membrane"
Improving ionic liquid tolerance in Saccharomyces cerevisiae through heterologous expression and directed evolution of an ILT1 homolog from Yarrowia lipolytica.
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Reed et al. overexpressed endogenous ScILT1/YDR090C and a Yarrowia lipolytica homolog in S. cerevisiae, then improved the heterologous YlILT1 by directed evolution for EMIM acetate tolerance.
"Here, we show that dominant
overexpression of an Ilt1p homolog (encoded by YlILT1/YALI0C04884) from the
IIL-tolerant yeast Yarrowia lipolytica confers an improvement in
1-ethyl-3-methylimidazolium acetate tolerance in Saccharomyces cerevisiae
compared to the endogenous Ilt1p (ScILT1/YDR090C)."
ILT1: inspected PAINT ancestry
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Actual accession-mapped ancestry was checked, independently of subfamily labels and donor counts.
"All four disputed inherited annotations originate at PTN001044753 and remain positive on target leaf PTN000415976."
Falcon literature report for yeast ILT1
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Comparative PQ-loop structures and characterized Ypq/PQLC2 relatives support a transporter hypothesis but are not target-specific biochemical measurements.
"the specific substrate and physiological role of YDR090C have **not yet been established directly** in the available primary literature"
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The report overlooked the dedicated 2018 target microscopy and tolerance experiments.
"No direct experimental localization data for YDR090C/ILT1 was identified in the primary literature."