FOL3 (YMR113W, Q12676) notes

• Dihydrofolate synthetase, EC 6.3.2.12 [UniProt:Q12676]. "dihydrofolate synthetase catalyzing the binding of the first glutamyl side chain to dihydropteroate yielding dihydrofolate is encoded by the YMR113w gene that we propose to rename FOL3" PMID:10799479
• "Mutant cells bearing a fol3 mutation require folinic acid for growth and have no dihydrofolate synthetase activity." PMID:10799479
• "folylpolyglutamate synthetase ... is encoded by the MET7 gene" PMID:10799479. The two glutamate-adding activities are therefore split between the paralogs FOL3 and MET7.
• A DHFS-deficient mutant was built and rescued by folinic acid PMID:11731153.
• Location: cytoplasm by GFP PMID:14562095.

Curation decisions

• The FPGS rows (GO:0004326, IBA and IEA) are marked over-annotated. This is paralog subfunctionalisation: the IBA's yeast experimental source is MET7.
• The mitochondrion IBA (from FPGS paralogs) is marked over-annotated.
• GO:0046901 (polyglutamate biosynthesis, UniPathway) is marked over-annotated.
• The folic acid metabolic and biosynthetic RCA rows are changed to GO:0006761.
• The folate cycle and chorismate rows are removed.