"Overexpression of either hsp26 or hsp27 extended the mean lifespan by 30%, and the flies also displayed increased stress resistance"

"This screen indeed identified 13 genes, some already known to be involved in longevity, plus candidate genes. Two of these, hsp26 and hsp27, were chosen to test for their effects on lifespan"

"these assays suggest that stress proteins, including Hsp23, Hsp26, Hsp83 and Frost as well as membrane-associated proteins may contribute to the cold hardening response"

"Microarray analysis to examine the changes in transcript abundance associated with cold hardening treatment...Quantitative RT-PCR was used to independently determine transcript abundance"

"A 5 M excess of Hsp23 and Hsp26 was required to obtain the same efficiency with either citrate synthase or luciferase as substrate"

"In an in vitro refolding assay with reticulocyte lysate, more than 50% of luciferase activity was recovered when heat denaturation was performed in the presence of Hsp22, 40% with Hsp27, and 30% with Hsp23 or Hsp26."

"These differences in luciferase reactivation efficiency seemed related to the ability of sHsps to bind their substrate at 42 degrees C, as revealed by sedimentation analysis of sHsp and luciferase on sucrose gradients"

"the 4 main sHsps of Drosophila share the ability to prevent heat-induced protein aggregation and are able to maintain proteins in a refoldable state, although with different efficiencies"

"We have identified several putative Sisyphus cargos, including DE-cadherin (also known as Shotgun) and the microtubule-linked proteins Katanin-60, EB1, Milton and aPKC"

"we performed comparative analyses with the sHSPs from other four insects whose complete genome sequences are available"

"sHSPs primarily have chaperone activity and reflect the response machine of organisms to some extreme stresses existing in environment"

"D. melanogaster has 11 sHSP genes...chromosome 3 of D. melanogaster (8 sHSP genes)"

"We have now devised a protocol to screen for substrates of this particular ubiquitin ligase. In a neuronal cell system, we find direct ubiquitination by Ube3a of three proteasome-related proteins Rpn10, Uch-L5, and CG8209, as well as of the ribosomal protein Rps10b."

"The four classical small HSPs (HSP22, HSP23, HSP26, and HSP27) were all highly induced after a heat shock"

"overexpression of the classical small HSPs (HSP23, HSP26, and HSP27) increased luciferase refolding"

"our results strongly suggest that the refolding capacity of D. melanogaster HSP27 and CG14207 is partially dependent on an intact HSP70 machine"

"We identified CG14207 as a novel and potent small HSP member that exclusively assisted in HSP70-dependent refolding of stress-denatured proteins. Furthermore, we report that HSP67BC, which has no role in protein refolding, was the most effective small HSP preventing toxic protein aggregation in an HSP70-independent manner."

"Both sHSPs immunoprecipitate together and the equilibrium between both chaperones is required for neuronal development and activity."

"The confocal images show an accumulation and colocalization of sHSP23 and sHSP26 throughout the NMJ but particularly intense in the synaptic buttons"

"we suggest that sHSP23 and sHSP26 together form a complex that promotes synapse formation in presynaptic neurons"

"We use an Hsp26-GFP-V5 fusion construct. sHSP23 was visualized using an anti-Hsp23...and sHSP26 was visualized using anti-V5"

"Pkm regulates expression and protein stability and participates in the establishment of synapse number during development."

"The network contains 32,668 interactions among 3,644 proteins, organized into 632 clusters representing putative functional modules."

"They play a crucial role in the maintenance of protein homeostasis, preventing nonspecific aggregation of the substrate protein in an ATP-independent manner"

"Hsp26, Hsp67Ba, Hsp23, and Hsp27 are highly expressed in early stage embryos (4–6 h after egg laying, AEL)"

"Hsp26 mRNA is present in nurse cells and later in the oocyte"

"Hsp26 is expressed in germline cell types including primary spermatocytes, and in some spermatogonia and spermatids"

"ubiquitous RNAi knockdown of six Drosophila sHsps (Hsp23, Hsp26, Hsp27, CG4461, l(2)efl, and CG14207) resulted in lethality"

"We then measured the activity of Hsp genes in Med15-silenced ovaries and found that, with the exception of the Hsp70Ba and Hsp26 genes, the expression of the examined Hsp genes was significantly reduced, however to varying degrees compared to that of the WT"

"They typically assemble as **dimers** (ACD-mediated) that serve as building blocks for **dynamic oligomers**, and they function primarily as **ATP-independent “holdase” chaperones**: they bind misfolded/unfolding proteins to prevent nonspecific aggregation and keep clients in a folding-competent state for subsequent refolding or processing by ATP-dependent chaperone systems."

"Hsp26 required approximately a **5-fold molar excess** to reach suppression levels achieved by Hsp22/Hsp27 near 1:1 ratios in citrate synthase aggregation assays."

"luciferase activity recovery after denaturation in the presence of Hsp26 was **35.7% ± 3.5%**, compared with **54.9% ± 2.8%** (Hsp22) and **42.8% ± 3.3%** (Hsp27)."

"Hsp26 is **predominantly cytosolic/cytoplasmic**, with a **granular cytosolic staining pattern** distinct from Hsp23; a **minor fraction** was also detected in the **nuclear matrix** of embryos and S2 cells."

"Hsp26 exists as multiple molecular forms: reports summarize the presence of **five isoforms**, and note that **three serines can be phosphorylated** and that **ubiquitination** of Hsp26 has been observed in fly neurons."

"It is described as highly expressed in **early embryos (4–6 h after egg laying)**, and enriched in **ovaries** and **testes**."

"ubiquitous RNAi knockdown of multiple sHsps including Hsp26 caused **lethality**, supporting that sHsps (including Hsp26) contribute indispensably to proteostasis and tissue robustness during development."

"**Hsp26 (and Hsp70Ba)** were explicit **exceptions** and did not show the basal reduction observed for other Hsp genes under those conditions."