Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
MUP-4 is a novel transmembrane protein with functions in epithelial cell adhesion in Caenorhabditis elegans.
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MUP-4 is a hemidesmosomal transmembrane protein required for attachment of the apical epidermis to the cuticle.
"In contrast, all mup-4 mutants (n = 14) showed a lesion between the apical hypodermal membrane and the cuticle."
Structural damage in the C. elegans epidermis causes release of STA-2 and induction of an innate immune response.
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Hemidesmosome disruption releases STA-2 and induces AMP transcription.
"The epidermis recognized structural damage through hemidesmosomes associated with a STAT-like protein, whose disruption led to detachment of STA-2 molecules from hemidesmosomes and transcription of AMPs."
C. elegans Hemidesmosomes Sense Collagen Damage to Trigger Innate Immune Response in the Epidermis.
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Damage to specific DPY collagens separates BLI-1 (a presumptive ligand) from MUP-4 and releases STA-2. Direct BLI-1/MUP-4 binding is not yet validated biochemically.
"However, the direct binding between BLI-1 and MUP-4 requires biochemical methods for further validation."