WDR35 (IFT121) notes

Summary

• IFT-A peripheral subunit PMID:27932497
• Ciliogenesis/localization: PMID:21473986
• COPI-like fold: PMID:21473986
• Membrane-protein entry: PMID:27806291; additional roles PMID:27806291
• Disease: SRTD7, CED2 (UniProt).

Curation decisions

• 4 protein binding IPI rows (IFT43/IFT122): REMOVE (uninformative; captured by part_of IFT-A).
• All other rows (IFT-A, cilium/axoneme/tip/basal body/centrosome, anterograde and retrograde IFT, cilium assembly, protein localization to cilium, protein carrier contributes_to) ACCEPT.
• Anterograde transport IDA from 40472089: accepted, deferring to curator (same caveat as IFT122).

HPA cilium atlas vs module role

• HPA v25: WDR35 is not in HPA (no antibody-based localization), so the atlas neither supports nor contradicts the module.
• Module: IFT-A subunit; retrograde IFT; IFT cargo adaptor; non-motile cilium.
• Assessment: consistent with the literature. core_functions = contributes to IFT-A cargo carrier activity (membrane-protein import), retrograde IFT, cilium assembly. Fu et al. show WDR35 is most prominently needed for membrane-protein entry, which supports the module's "cargo adaptor" label more than retrograde transport alone.

Deep research

See below.
Falcon deep research succeeded (WDR35-deep-research-falcon.md). Points used:
- [file:human/WDR35/WDR35-deep-research-falcon.md "Reintroducing wild-type WDR35 rescued IFT88 retrograde transport and ARL13B entry."]
- WDR35-dependent periciliary vesicle coat (Quidwai et al. 2021, mouse; not cached): the IFT139-IFT121-IFT43 trimer binds phosphatidic acid. This supports the COPI-like-coat question in suggested_questions; no annotation proposed.