Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria.
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MWFE/NDUFA1 is a 70-aa accessory subunit of mammalian Complex I, not one of the 14 core subunits, and is not itself catalytic.
"it is not one of the 14 polypeptides making up the core complex I"
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MWFE is absolutely essential for an active Complex I; its loss reduces activity to <10%, restored by NDUFA1 cDNA complementation.
"the MWFE polypeptide is absolutely essential for an active complex I in mammals."
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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NDUFA1 is identified by mass spectrometry as a subunit of immunopurified human Complex I (NADH dehydrogenase).
"we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied"
Novel localization of OCTN1, an organic cation/carnitine transporter, to mammalian mitochondria.
Identification of mitochondrial complex I assembly intermediates by tracing tagged NDUFS3 demonstrates the entry point of mitochondrial subunits.
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Complex I assembly proceeds stepwise through discrete subcomplexes; NDUFA1 is a Complex I subunit assayed in this membrane/assembly context.
"we here provide direct evidence for the stepwise assembly of CI."
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Human Complex I comprises 45 subunits; 14 core subunits are shared with bacteria and 31 are accessory subunits.
"Bacterial and human complex I share 14 core subunits that are essential for enzymatic function"
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25 of the accessory subunits are strictly required for assembly of a functional Complex I, and loss of a subunit destabilizes co-module subunits.
"We show that 25 subunits are strictly required for assembly of a functional complex"
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human respiratory megacomplex reveals the precise assignment of individual Complex I subunits, including NDUFA1.
"reveals the precise assignment of individual subunits of human CI"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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OXPHOS complexes contain core catalytic proteins plus many supernumerary subunits with essential roles in assembly, regulation and stability; complexes I, III and IV form respiratory supercomplexes.
"a large number of 'supernumerary' subunits that play essential roles in assembly, regulation and stability."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Complex I is an inner-membrane multiprotein complex whose main function is electron transport from NADH to ubiquinone coupled to proton translocation.
"Its main function is the transport of electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space."
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex