The literature distinguishes reconstituted H1.4 arrays from cellular mitotic experiments. Heterochromatin preference is compatible with euchromatin occupancy. Modified histone pools do not define all H1.4 localization.
All incoming source identifiers, qualifiers and extension conjunctions remain intact. No NEW annotation is proposed. Availability of an abstract or structured assay record is not a claim to having read unavailable full text.
The 52 incoming records map to 51 schema contexts. Raw records 4 and 5 differ only in source-assignment, date and opaque identifier; both complete records and both manual decisions remain in the evidence map, with one shared current review. All 17 extension atoms are preserved.
Three independently reviewed slices were integrated without action changes. The stored decisions are 19 ACCEPT, 9 TOO_GENERAL, 4 KEEP_AS_NON_CORE, 18 REMOVE and 1 UNDECIDED, with no NEW annotations. Native acquisition, computed status, history, affected checks and rendering remain pending.
The preceding pending workflow statement is superseded by actual native results. Genuine helpers produced the 52-row GOA and 51-context PENDING seed, matching the reviewed input bytes. All 17 extension atoms and the sole two-record provenance collision are preserved. The 33 inherited sources remain exact; 22 planned source artifacts were acquired. Actual titles, cache flags and conditional quotes were reconciled. Affected gene, strict GOA, reference, status and history checks passed. Computed status is DRAFT with 1 reported warnings. Actual reference checked/skipped counts, rendered output and family/cache conservation results are retained separately.
H1.4 occupancy spans chromatin states. Human fetal H1s-4 is relatively depleted from active chromatin, while H1.4 K34 acetylation is enriched at active promoters. The historical H1s-4 identity is resolved by HGNC:4718. [PMID:10997781; PMID:22465951]
K26 methylation supports HP1 binding, whereas neighboring S27 phosphorylation prevents it. Chromatin tethering remains a proposed consequence. PMID:16127177
The imaging study reports "H1.0 and H1.4 were distributed throughout the nucleus." This broad pool does not exclude HPA nuclear-speck staining; phospho-antibody cross-reactivity limits separate speck confirmation. PMID:38530350
Rahman-associated frameshifts produce altered tails; their consequences should not be equated with ordinary H1.4 loss. The functional study expressed human constructs in rat hippocampal neurons, and overgrowth was not obligatory among affected individuals. PMID:34788807
The source assessments now record the roles and limits of the fourteen previously unannotated references. Their inclusion does not require additional GO assertions. Existing journal entries, raw records, qualifiers, extensions and source bytes are retained. This private follow-up has one action change: stored row 0 TOO_GENERAL to ACCEPT; all other actions and the core remain unchanged. Native history, validation and rendering remain pending.
The follow-up passed default validation, strict GOA comparison, reference validation, computed-status audit, history validation and rendering. The repository computed COMPLETE with no validation warnings. Reference validation checked five snippets, skipped none, checked 52 titles and found zero issues. All original source, downloaded input and GO cache bytes remain unchanged.
Applied the standing ClinGen curation instruction: sixteen supported generic-binding rows are now KEEP_AS_NON_CORE and two are UNDECIDED. This supersedes the earlier one-action scope statement; all preceding notes, raw provenance, partners, extensions and core functions remain preserved.
PMID:35271311 and PMID:36950384 remain unresolved because their target-specific results were not independently recovered; missing verification is not negative interaction evidence.
Continuation checks passed: strict GOA preserved every schema context; reference validation checked five snippets, skipped none, checked 52 titles and reported zero issues. The computed status is DRAFT with sixteen protein-binding policy warnings. Those warnings reflect the repository-wide informational-exclusion rule; the standing ClinGen instruction is deliberately retained. The earlier COMPLETE result remains historical.