Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Supersensitivity to mu-opioid receptor-mediated inhibition of the adenylyl cyclase pathway involves pertussis toxin-resistant Galpha protein subunits.
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Mu-opioid receptor agonists stimulate [35S]GTPgammaS binding and inhibit forskolin-stimulated adenylyl cyclase activity via pertussis-toxin-sensitive Galpha(i/o) subunits.
"In control animals, the mu-opioid selective agonists, sufentanil and DAMGO, stimulated [35S]5'-(gamma-thio)-triphosphate ([35S]GTP gamma S) binding and inhibited forskolin-stimulated adenylyl cyclase activity, through a mechanism involving pertussis toxin (PTX) sensitive G alpha(i/o) subunits."
Crystal structure of the µ-opioid receptor bound to a morphinan antagonist.
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The mu-opioid receptor couples predominantly to Gi, the inhibitory G protein for adenylyl cyclase.
"The μOR couples predominantly to Gi, the inhibitory G protein for adenylyl cyclase. μOR signaling through Gi is responsible for its analgesic properties4."
Ligand recognition and allosteric modulation of the human MRGPRX1 receptor.
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Cryo-EM structures of human MRGPRX1-Gq bound to PENK-derived BAM8-22 reveal the mechanism of pruritogenic peptide recognition.
"Here we report three high-resolution cryogenic electron microscopy structures of MRGPRX1-Gαq in complex with BAM8-22 alone, with BAM8-22 and ML382 simultaneously as well as with a synthetic agonist compound-16."
Ligand recognition and G protein coupling of the human itch receptor MRGPRX1.
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Human MRGPRX1 is an itch receptor activated by the endogenous PENK-derived cleavage product BAM8-22.
"In particular, MRGPRX1, one of the itch receptors in humans, is able to respond to endogenous peptides, such as γ2-melanocyte-stimulating hormone (γ2-MSH)7, bovine adrenal medulla peptide (BAM) 8-228, and the antimalarial drug chloroquine (CQ)6, which has an intolerant itch side effect."
Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA.
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Cloning of human pheochromocytoma proenkephalin cDNA revealed a 267-residue precursor containing six Met-enkephalin sequences and one Leu-enkephalin sequence.
"The corresponding amino acid sequence shows that the precursor is 267 amino acids long and contains six interspersed Met-enkephalin sequences and one Leu-enkephalin sequence."
Opioid receptors bind opioid peptides
Liganded Gi-activating GPCR acts as a GEF for Gi
The Ligand:GPCR:Gi complex dissociates
Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
FAM20C phosphorylates FAM20C substrates