Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade
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CAHS proteins identified by mass spectrometry in heat-soluble protein fractions
"Our heat-soluble proteomics identified five abundant heat-soluble proteins"
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GFP-fusion experiments show cytoplasmic localization with weak nuclear signal
"We named them Cytoplasmic Abundant Heat Soluble (CAHS) and Secretory Abundant Heat Soluble (SAHS) protein families, according to their localization"
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CAHS proteins contain repeating 19-mer peptide motifs suggesting molecular shield function
"Two conserved repeats of 19-mer motifs in CAHS proteins were capable to form amphiphilic stripes in alpha-helices, suggesting their roles as molecular shield in water-deficient condition"
Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
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16 CAHS genes found in R. varieornatus genome
"We found significant expansion of these tardigrade-unique protein families, as 16 CAHS genes and 13 SAHS genes in our assembly, whereas no counterparts were found in other phyla"
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CAHS genes are constitutively and abundantly expressed
"These abundantly expressed proteins included previously identified tardigrade-unique heat-soluble proteins, CAHS and SAHS"
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CAHS proteins proposed to be involved in protection of biomolecules during desiccation
"previously identified tardigrade-unique heat-soluble proteins, CAHS and SAHS, both of which maintain solubility even after heat treatment and are proposed to be involved in the protection of biomolecules during desiccation"
Reconsidering the glass transition hypothesis of intrinsically unstructured CAHS proteins in desiccation tolerance of tardigrades