Annotation inferences using phylogenetic trees

IBA annotations for A0A3B6RKV1 are inferred from orthologs including AT5G06550 (Arabidopsis JMJ22/PKDM7D), Q386V9, and Q96S16 via PANTHER phylogenetic trees.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

PMID:22483719

Control of seed germination by light-induced histone arginine demethylation activity.

JMJ20 and JMJ22 act redundantly as positive regulators of seed germination. Upon PHYB activation, JMJ20/JMJ22 are derepressed, resulting in increased gibberellic acid levels through the removal of repressive histone arginine methylations (H4R3me2) at GA3ox1/GA3ox2, which in turn promotes seed germination.

file:WHEAT/A0A3B6RKV1/A0A3B6RKV1-deep-research-falcon.md

Deep research report for A0A3B6RKV1 (LOC123148885) in Triticum aestivum

Note: the falcon deep research report originally classified LOC123148885 in the KDM5/JARID1 subfamily; this was corrected by OpenScientist investigation (see below) to JMJD6 subfamily based on domain architecture, PANTHER, InterPro, and NCBI orthology evidence.
Wang et al. (2022) identified 24 JmjC genes in wheat. 18 of 24 are predicted to localize to the nucleus. Wheat JmjC genes show dynamic expression under drought stress.
The protein contains both a JmjC histone demethylase domain and an F-box domain, suggesting potential dual functionality in epigenetic regulation and SCF-mediated protein degradation.

file:WHEAT/A0A3B6RKV1/A0A3B6RKV1-hypotheses/core-function-1-go-0032452/openscientist.md

OpenScientist deep research report on core function hypothesis for A0A3B6RKV1 histone demethylase activity

CRITICAL CORRECTION: A0A3B6RKV1 belongs to the JMJD6 subfamily (PANTHER PTHR12480:SF21, InterPro IPR050910), not KDM5/JARID1. Its domain architecture (F-box + JmjC, 511 aa) is incompatible with KDM5 (requires JmjN, ARID, PHD, FYR; 800-1200+ aa). NCBI orthology identifies At5g06550 (JMJ22) as the Arabidopsis ortholog.
AtJMJ22 is an experimentally characterized H4R3me2 arginine demethylase (PMID:22483719), not an H3K4 lysine demethylase. The protein's substrate is histone H4 arginine 3 dimethyl marks, not histone H3 lysine 4.
A0A3B6RKV1 exists as a complete homeologous triad: 7A (A0A3B6RKV1), 7B (A0A3B6SQ95), 7D (A0A3B6TWS8), all 511 aa, 96-98% identity, with perfectly conserved Fe(II)-binding catalytic triad (His330-Asp332-His362).
The UniProt Similarity field stating JARID1 family membership is an automated annotation error contradicted by PANTHER, InterPro, NCBI, and domain architecture.