Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
A novel acylglycerol kinase that produces lysophosphatidic acid modulates cross talk with EGFR in prostate cancer cells.
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Human AGK phosphorylates monoacylglycerol and diacylglycerol to generate LPA and PA.
"(AGK), that phosphorylates monoacylglycerol and diacylglycerol to form LPA and PA, respectively"
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The same study did not support ceramide or sphingosine as AGK substrates.
"Significant phosphorylated products were only detected with monoacylglycerols and diacylglycerols as substrates, but not with any other lipid tested, including ceramide and sphingosine"
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AGK localizes to mitochondria in microscopy and fractionation assays.
"Confocal microscopy and subcellular fractionation suggest that AGK is localized to the mitochondria"
Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.
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This ceramide kinase study argues against assigning ceramide kinase activity to the multiple lipid kinase now curated as AGK.
"No evidence for phosphorylation of ceramide by the recently described multiple lipid kinase was found"
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The study notes that the multiple lipid kinase is mitochondrial.
"The latter kinase is localized in the mitochondria"
Lack of the mitochondrial protein acylglycerol kinase causes Sengers syndrome.
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Biallelic loss-of-function AGK variants cause Sengers syndrome (congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, lactic acidosis), and AGK loss decreases the adenine nucleotide translocator in the inner mitochondrial membrane in muscle.
Architecture of the human interactome defines protein communities and disease networks.
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
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AGK is a constituent of the TIM22 complex in the mitochondrial inner membrane.
"we have identified AGK as a constituent of the TIM22 complex in the mitochondrial inner membrane"
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AGK supports import of a subset of multi-spanning mitochondrial membrane proteins.
"AGK assembles with TIMM22 and TIMM29 and supports the import of a subset of multi-spanning membrane proteins"
Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.
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AGK is a human TIM22 protein import complex subunit.
"we identified AGK as a subunit of the mitochondrial TIM22 protein import complex"
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AGK supports TIM22 integrity and carrier-protein import independently of kinase activity.
"AGK functions in a kinase-independent manner to maintain the integrity of the TIM22 complex, where it facilitates the import and assembly of mitochondrial carrier proteins"
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
The TIM22 complex mediates the import of sideroflexins and is required for efficient mitochondrial one-carbon metabolism.
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AGK-containing TIM22 imports sideroflexin (SFXN) proteins as a novel substrate class beyond SLC25 carriers, and AGK loss impairs SFXN biogenesis, linking AGK deficiency to dysregulated mitochondrial one-carbon metabolism.
Cryo-EM structure of the human mitochondrial translocase TIM22 complex.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Mitochondrial phospholipid metabolism in health and disease.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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The mitochondrial proteome paper explicitly discusses long-lived acylglycerol kinase associated with TIMM22.
"whereas the associated acylglycerol kinase, mutations of which cause Sengers syndrome"
LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma.
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SMIM26 interacts with AGK in a renal-cell carcinoma context.
"SMIM26, but not LINC00493, suppresses ccRCC growth and metastatic lung colonization by interacting with acylglycerol kinase (AGK)"
AGK:Mg2+ phosphorylates MAG, DAG
BRAF and RAF fusion mutant dimers are phosphorylated
Dissociation of BRAF/RAF fusion complex
p-BRAF and RAF fusion dimers phosphorylate MAP2Ks
p-BRAF and RAF fusion dimers bind MAP2Ks and MAPKs
MAPKs are phosphorylated downstream of BRAF and RAF fusion dimers
UniProt record for human AGK
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UniProt summarizes AGK lipid kinase and TIM22 complex roles.
"Independently of its lipid kinase activity, acts as a component of the TIM22 complex"
OpenScientist hypothesis report for AGK ceramide kinase activity
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OpenScientist classified AGK ceramide kinase activity as over-annotated and recommended removing GO:0001729.
"The assignment of ceramide kinase activity (GO:0001729) to human AGK (Q53H12) is not supported by direct experimental evidence and is contradicted by the available biochemical literature."
Manual AGK Proteostasis PN review notes
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Manual fallback notes document the failed Falcon/fallback provider run and the conservative PN projection decision.
"The strongest PN-relevant role is the TIM22 import-complex role, not a generic protein-transport assertion."
Proteostasis PN projected annotations report
MuLK, a eukaryotic multi-substrate lipid kinase.
Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.
Mitochondrial citrate synthase crystals: novel finding in Sengers syndrome caused by acylglycerol kinase (AGK) mutations.
Characterization of a Novel Splicing Variant in Acylglycerol Kinase (AGK) Associated with Fatal Sengers Syndrome.
Case Report: Sengers syndrome caused by a novel 7.6 kb AGK deletion misdiagnosed as isolated congenital cataract.
The role of AGK in thrombocytopoiesis and possible therapeutic strategies.
Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase.
Role of acylglycerol kinase in LPA-induced IL-8 secretion and transactivation of epidermal growth factor-receptor in human bronchial epithelial cells.
A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration.
Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as biomarkers in diabetic retinopathy.
Atypical Presentation of Sengers Syndrome: A Novel Mutation Revealed with Postmortem Genetic Testing.
Acylglycerol Kinase-Targeted Therapies in Oncology.
AGK Potentiates Arterial Thrombosis by Affecting Talin-1 and αIIbβ3-Mediated Bidirectional Signaling Pathway.
Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2.
Aberrantly Expressed Mitochondrial Lipid Kinase, AGK, Activates JAK2-Histone H3 Axis and BCR Signal: A Mechanistic Study with Implication in CLL Therapy.
Novel c.221+1dup pathogenic variant in AGK gene linked to Sengers syndrome.
Ceramide kinase suppresses ferroptosis and protects against alcohol-associated liver disease through the p38 MAPK-HSPB1 pathway.
Role of Ceramide Kinase/C1P in the Regulation of Cell Growth and Survival.
Characterization of Ceramide Kinase from Basolateral Membranes of Kidney Proximal Tubules: Kinetics, Physicochemical Requirements, and Physiological Relevance.
Oocyte-specific acylglycerol kinase loss leads to infertility in mice via mitochondrial dysfunction.
Acylglycerol kinase contributes to cell proliferation by activating NF‑κB signaling pathway in pancreatic cancer.
Ceramide kinase/ceramide 1-phosphate signaling regulates LC3B expression and autophagosome formation.
Acylglycerol Kinase Sensitizes Glioblastoma to Temozolomide via Limiting Mitochondrial Damage Related Cellular Senescence.
AGK(1-33)InsW-p-BRAF(328-766) fusion [cytosol]