Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component
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Demonstrates CelS has exoglucanase activity with preference for crystalline cellulose
"The recombinant CelS (rCelS), the most abundant catalytic subunit of the Clostridium thermocellum cellulosome, displayed typical exoglucanase characteristics, including (i) a preference for amorphous or crystalline cellulose over carboxymethyl cellulose"
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Provides evidence for EC 3.2.1.176 classification
"The hydrolysis products from crystalline cellulose were cellobiose and cellotriose at a ratio of 5:1"
Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component
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Original cloning and sequencing of celS gene
"we cloned and sequenced the gene (celS) coding for the SS (CelS) protein by using a degenerate, inosine-containing oligonucleotide probe whose sequence was derived from the N-terminal amino acid sequence of the CelS protein"
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Identifies signal peptide and protein sequence
"A putative signal peptide of 27 amino acid residues was adjacent to the N terminus of the CelS protein. The predicted molecular weight of the secreted protein was 80,670"
Product inhibition of the recombinant CelS, an exoglucanase component of the Clostridium thermocellum cellulosome
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Shows CelS is inhibited by cellobiose and lactose, but not by glucose
"The rCelS activity on cellopentaose was strongly inhibited by cellobiose. The rCelS activity was also inhibited by lactose. Glucose was only marginally inhibitory"
UniProt entry A3DH67 for CelS
Deep research review of CelS function and literature