Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Cloning of cDNAs encoding isopropylmalate dehydrogenase from Arabidopsis thaliana and accumulation patterns of their transcripts.
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AtIMD1 complements yeast leu2; putative plastid transit peptide.
"AtIMD1, AtIMD2, and AtIMD3 were able to complement a leu2 mutant of yeast, suggesting that these genes encode functional IPMDH."
The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase.
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atimd1 knockouts have reduced Met-derived glucosinolates; gene named MAM-D1.
"In this study we found that knocking out either of the candidate genes, AtleuC1 or AtIMD1 , reduced the level of Met-GSLs, indicating that both genes are actually involved in Met-GSL biosynthesis."
A redox-active isopropylmalate dehydrogenase functions in the biosynthesis of glucosinolates and leucine in Arabidopsis.
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IMDH1 functions in both glucosinolate and leucine biosynthesis; mutant has reduced leucine and C4+ glucosinolates; stromal; redox regulated.
"We report a detailed functional characterization of an Arabidopsis isopropylmalate dehydrogenase (AtIPMDH1) that is involved in both glucosinolate biosynthesis and leucine biosynthesis."
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Functional characterization of Arabidopsis thaliana isopropylmalate dehydrogenases reveals their important roles in gametophyte development.
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IPMDH2/3 have higher 3-IPM activity than IPMDH1 and are the principal Leu-pathway IPMDHs, essential for gametophyte development.
"IPMDH2 and IPMDH3 proteins exhibited significantly higher activity toward 3-IPM than IPMDH1, which is indicative of a pivotal role in leucine biosynthesis."
Structural and functional evolution of isopropylmalate dehydrogenases in the leucine and glucosinolate pathways of Arabidopsis thaliana.
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IMDH1 is ~500-fold more efficient with the glucosinolate substrate than IMDH2/3; Phe-137 determines specificity in vitro and in vivo.
"AtIPMDH1 accepts both substrates with comparable k cat / K m values, but was ∼500-fold more efficient with the glucosinolate substrate than the other two isoforms."
A proteomic strategy for global analysis of plant protein complexes.
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation.
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Chloroplast proteomics detected all three IPMDHs in plastid fractions.
"which also reported BCAT3, MAM1 and all three potential isopropylmalate dehydrogenases in protein fractions from plastids"
Protein complex formation in methionine chain-elongation and leucine biosynthesis.
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IMDH1 interacts with the IPMI large subunit LeuC (Y2H, BiFC, Co-IP); IMDH1 is the major Met chain-elongation IPMDH.
"we identified an unprecedented interaction of the IPMI large subunit (LeuC) with IPMDH1 or IPMDH2, and a weaker interaction with IPMDH3 in both Y2H and BiFC experiments."
Structure and Mechanism of Isopropylmalate Dehydrogenase from Arabidopsis thaliana: INSIGHTS ON LEUCINE AND ALIPHATIC GLUCOSINOLATE BIOSYNTHESIS.
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Structures of AtIPMDH2 define a common mechanism for Leu- and glucosinolate-pathway IPMDHs.
"This study suggests that IPMDH-like enzymes in both leucine and glucosinolate biosynthesis pathways use a common mechanism"
Biosynthesis of glucosinolates--gene discovery and beyond.
UniProt record for Arabidopsis IMDH1 (Q9FMT1)