The algT (algU) gene of Pseudomonas aeruginosa, a key regulator involved in alginate biosynthesis, encodes an alternative sigma factor (sigma E).
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Purified AlgT with E. coli core RNAP initiates transcription of the algT promoter in vitro, dependent on the -35 region.
"The purified protein, in the presence of E. coli RNA polymerase core enzyme, was able to initiate transcription of an algT promoter"
Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB, and algD reveals a hierarchy of alginate gene expression which is modulated by algT.
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algT insertions abolish transcription initiation at the algR and algD promoters.
"Expression of algR and algD was abolished in P. aeruginosa strains containing algT::Tn501 insertions because of lack of transcription initiation at the algR and algD promoters"
Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.
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algZ is transcribed from a consensus AlgT promoter whose -35/-10 mutations reduce expression.
"Most likely, AlgT is directly involved in algZ promoter recognition and transcription initiation since a promoter that is similar to a consensus AlgT promoter was identified upstream of the mapped transcription start site"
Global genomic analysis of AlgU (sigma(E))-dependent promoters (sigmulon) in Pseudomonas aeruginosa and implications for inflammatory processes in cystic fibrosis.
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Ten new AlgU-dependent promoters were mapped by comparing algU+ and algU mutant RNA.
"A total of 10 new AlgU-dependent promoters were identified, and the corresponding mRNA start sites were mapped"
Use of cell wall stress to characterize sigma 22 (AlgT/U) activation by regulated proteolysis and its regulon in Pseudomonas aeruginosa.
MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa: involvement in chlorhexidine resistance and induction by membrane-damaging agents dependent upon the AlgU stress response sigma factor.
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Induction of mexCD-oprJ by chlorhexidine and membrane-active agents depends on AlgU.
"induce expression of the mexCD-oprJ multidrug efflux operon, dependent upon the AlgU stress response sigma factor"
Microbial pathogenesis in cystic fibrosis: co-ordinate regulation of heat-shock response and conversion to mucoidy in Pseudomonas aeruginosa.
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AlgU-RNAP transcribes rpoH from its P3 promoter, induced by heat shock.
"was found to be transcribed by AIgU containing RNA polymerase from one of its promoters (P3) identified in this study"
The sigma factor AlgU plays a key role in formation of robust biofilms by nonmucoid Pseudomonas aeruginosa.
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algU mutant is defective in attachment and robust biofilm; psl/lec control is probably indirect via ppyR.
"Promoter mapping indicated that AlgU is probably not directly responsible for transcription of the psl operon and the lec genes"
The alternative sigma factor AlgT represses Pseudomonas aeruginosa flagellum biosynthesis by inhibiting expression of fleQ.
Analysis of promoters controlled by the putative sigma factor AlgU regulating conversion to mucoidy in Pseudomonas aeruginosa: relationship to sigma E and stress response.
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algU mutants are more sensitive to paraquat and heat.
"The algU mutants, irrespective of the mucoid status of the parental strains, displayed increased sensitivity to killing by paraquat"
Extracytoplasmic function sigma factors in Pseudomonas aeruginosa.
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Review grouping the P. aeruginosa ECF sigma factors by function.
"the iron starvation σ (PvdS, FpvI, HasI, FecI, FecI2 and others), the cell wall stress ECFσ AlgU, SigX and SbrI, and the unorthodox σVreI involved in the expression of virulence"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
UniProt entry for algU (PA0762)