Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4).
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Original cloning of human ACSL4/FACL4 from brain library with substrate preference for arachidonic acid
"The cDNA encodes a functional long-chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate"
FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation.
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FACL4/ACSL4 mutations cause X-linked intellectual disability with reduced enzymatic activity
"Analysis of enzymatic activity in lymphoblastoid cell lines from affected individuals of both families revealed low levels compared with normal cells"
A third MRX family (MRX68) is the result of mutation in the long chain fatty acid-CoA ligase 4 (FACL4) gene: proposal of a rapid enzymatic assay for screening mentally retarded patients.
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The P375L human FACL4 variant markedly reduces enzyme activity.
"changes a highly conserved proline into a leucine (p.P375L) in the first luciferase domain, which markedly reduces the enzymatic activity"
Identification of major proteins in the lipid droplet-enriched fraction isolated from the human hepatocyte cell line HuH7.
Long-chain acyl-CoA synthetase 4 modulates prostaglandin E₂ release from human arterial smooth muscle cells.
The Sjögren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
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ACSL family members involved in sphingolipid-to-glycerolipid metabolic pathway
"yeast Faa1 and Faa4 and mammalian ACSL family members are acyl-CoA synthetases involved in the sphingolipid-to-glycerolipid metabolic pathway"
Autophagosomes form at ER-mitochondria contact sites.
Diminished acyl-CoA synthetase isoform 4 activity in INS 832/13 cells reduces cellular epoxyeicosatrienoic acid levels and results in impaired glucose-stimulated insulin secretion.
Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
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Human ACSL4 belongs to the enzymes that restore the deficient sphingolipid-to-glycerophospholipid pathway in yeast.
"in addition to the previously identified ACSL family members (ACSL1, 3, 4, 5, and 6), we found that ACSVL1, ACSVL4, and ACSBG1 also restored metabolism"
ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition.
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ACSL4 supplies membrane PUFAs and promotes ferroptosis sensitivity in the studied models.
"Mechanistically, ACSL4 enriched cellular membranes with long polyunsaturated ω6 fatty acids"
Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4.
Histone lysine crotonylation accelerates ACSL4-mediated ferroptosis of keratinocytes via modulating autophagy in diabetic wound healing.
Fibroblast lipid metabolism through ACSL4 regulates epithelial sensitivity to ferroptosis in IBD.
Analysis on the Substrate Specificity of Recombinant Human Acyl-CoA Synthetase ACSL4 Variants.
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Both ACSL4 variants prefer HUFAs including DHA, adrenic acid, EPA, and AA
"both ACSL4 variants preferred various kinds of highly unsaturated fatty acids (HUFAs), including docosahexaenoic acid (DHA), adrenic acid (docosatetraenoic acid) and eicosapentaenoic acid (EPA), as well as AA as a substrate"
PKCβII phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis.
Tumor-repopulating cells evade ferroptosis via PCK2-dependent phospholipid remodeling.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Defining the membrane proteome of NK cells.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Intracellular metabolism of fatty acids regulates insulin secretion
ACSL3,4 ligates coenzyme A (CoA-SH) to a long chain fatty acid yielding fatty acyl-coenzyme A
ACSL3,4 ligate CoA to AA to form AA-CoA
Synthesis of very long-chain fatty acyl-CoAs
Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction.
Cyberian deep research review on ACSL4 function
A novel arachidonate-preferring acyl-CoA synthetase is present in steroidogenic cells of the rat adrenal, ovary, and testis.
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Purified rat ACS4 preferentially activates arachidonate and eicosapentaenoate, with lower affinity for palmitate.
"the enzyme has a high affinity for arachidonate and eicosapentaenoate and low affinity for palmitate"
Role of acyl-CoA synthetase ACSL4 in arachidonic acid metabolism.
Analyses of mental dysfunction-related ACSl4 in Drosophila reveal its requirement for Dpp/BMP production and visual wiring in the brain.
Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids.
The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells.
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A minor endogenous ACSL4 pool was detected in isolated MAM fractions from MCF-7 cells, with additional ACSL4 in non-MAM ER.
"only a minor fraction of ACSL4 was present in the isolated MAM fraction with the bulk of the cellular compliment found in the cytoplasmic fraction"
Tissue-Specific Ablation of ACSL4 Results in Disturbed Steroidogenesis.
Mutagenesis of rat acyl-CoA synthetase 4 indicates amino acids that contribute to fatty acid binding.
Rosiglitazone inhibits acyl-CoA synthetase activity and fatty acid partitioning to diacylglycerol and triacylglycerol via a peroxisome proliferator-activated receptor-gamma-independent mechanism in human arterial smooth muscle cells and macrophages.
Involvement of ACSL in local synthesis of neutral lipids in cytoplasmic lipid droplets in human hepatocyte HuH7.
Intellectual disability, midface hypoplasia, facial hypotonia, and Alport syndrome are associated with a deletion in Xq22.3.
Functional interaction between acyl-CoA synthetase 4, lipooxygenases and cyclooxygenase-2 in the aggressive phenotype of breast cancer cells.
Valproate uncompetitively inhibits arachidonic acid acylation by rat acyl-CoA synthetase 4: relevance to valproate's efficacy against bipolar disorder.
Fragile X and X-linked intellectual disability: four decades of discovery.
Propylisopropylacetic acid (PIA), a constitutional isomer of valproic acid, uncompetitively inhibits arachidonic acid acylation by rat acyl-CoA synthetase 4: a potential drug for bipolar disorder.
Xq22.3-q23 deletion including ACSL4 in a patient with intellectual disability.
Role of Protein Phosphorylation and Tyrosine Phosphatases in the Adrenal Regulation of Steroid Synthesis and Mitochondrial Function.
Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis.
ACSL4 reprograms fatty acid metabolism in hepatocellular carcinoma via c-Myc/SREBP1 pathway.
Mitochondria-Associated Endoplasmic Reticulum Membranes in Breast Cancer.
Protein Lipidation by Palmitoylation and Myristoylation in Cancer.
Insights into the post-translational modification and its emerging role in shaping the tumor microenvironment.
ACSL4 as a Potential Target and Biomarker for Anticancer: From Molecular Mechanisms to Clinical Therapeutics.
CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection.
ACSL4 and the lipoxygenases 15/15B are pivotal for ferroptosis induced by iron and PUFA dyshomeostasis in dopaminergic neurons.
Specificity protein 1-mediated ACSL4 transcription promoted the osteoarthritis progression through suppressing the ferroptosis of chondrocytes.
Post-translational palmitoylation of metabolic proteins.
LPCAT3 Is Transcriptionally Regulated by YAP/ZEB/EP300 and Collaborates with ACSL4 and YAP to Determine Ferroptosis Sensitivity.
New insights into signal transduction pathways in adrenal steroidogenesis: role of mitochondrial fusion, lipid mediators, and MAPK phosphatases.
The ACSL4 Network Regulates Cell Death and Autophagy in Diseases.
ACSL4-Mediated Ferroptosis and Its Potential Role in Central Nervous System Diseases and Injuries.
Acyl-CoA synthase ACSL4: an essential target in ferroptosis and fatty acid metabolism.
Rosiglitazone inhibits acyl-CoA synthetase long-chain family number 4 and improves secondary brain injury in a rat model of surgical brain injury.
ACSL4 inhibition prevents macrophage ferroptosis and alleviates fibrosis in bleomycin-induced systemic sclerosis model.
ACSL4-Mediated Membrane Phospholipid Remodeling Induces Integrin β1 Activation to Facilitate Triple-Negative Breast Cancer Metastasis.
Identification of a targeted ACSL4 inhibitor to treat ferroptosis-related diseases.
ACSL4 mediates inflammatory bowel disease and contributes to LPS-induced intestinal epithelial cell dysfunction by activating ferroptosis and inflammation.
The role of ACSL4 in stroke: mechanisms and potential therapeutic target.
Cerebral ischemia-reperfusion injury: mechanisms and promising therapies.
ACSL4 at the helm of the lipid peroxidation ship: a deep-sea exploration towards ferroptosis.
Long-chain acyl-CoA synthetases: biological functions, diseases and therapeutic targets.
From pathogenesis to treatment: the emerging role of ferroptosis in Parkinson's disease.
Beyond oxidative stress: Ferroptosis as a novel orchestrator in neurodegenerative disorders.
Correction: Insights into the post-translational modification and its emerging role in shaping the tumor microenvironment.